JaLCDOI 10.18926/AMO/30332
FullText URL fulltext.pdf
Author Manabe, Yuichi| Tsubota, Teruhiko| Haruta, Yuro| Kataoka, Kiyoshi| Okazaki, Morihiro| Haisa, Shigeru| Nakamura, Kunihiko| Kimura, Ikuro|
Abstract <p>Macromomycin (MCR), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-HLA IgG1 antibody (H-1) in the presence of carbodiimide. The resulting mixture was fractionated with a Sephadex G-200 column. The first and second fractions were shown to contain MCR-(H-1) conjugate by the elution profile, as well as by the Sarcina lutea growth inhibition assay and Ouchterlony double-diffusion method. A membrane immunofluorescence test with anti-MCR and anti-mouse IgG antibodies demonstrated specific localization of MCR-(H-1) on the surface of HLA-bearing NALL -1 cells. MCR-(H-1) inhibited the growth of HLA-lacking NS-1 cells statistically less effectively than MCR alone (p less than 0.01). On the other hand, the conjugate and free MCR equally inhibited the growth and 3H-TdR incorporation of HLA-bearing NALL -1 cells. These results indicate that the antibody-bound MCR retained both MCR and antibody activities, and thus exerted antibody-targeting MCR cytotoxicity in vitro.</p>
Keywords macromomycin(MCR) anti-HLA monoclonal antibody carbodiimide
Amo Type Article
Published Date 1984-04
Publication Title Acta Medica Okayama
Volume volume38
Issue issue2
Publisher Okayama University Medical School
Start Page 117
End Page 124
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6375268
Web of Science KeyUT A1984SN81800003