このエントリーをはてなブックマークに追加
ID 54549
FullText URL
Author
Ohtsuki, Takashi Department of Medical Bioengineering, Okayama University
Miki, Shunya Department of Medical Bioengineering, Okayama University
Kobayashi, Shouhei Advanced ICT Research Institute Kobe, NICT
Haraguchi, Tokuko Advanced ICT Research Institute Kobe, NICT
Nakata, Eiji Institute of Advanced Energy, Kyoto University
Hirakawa, Kazutaka Department of Applied Chemistry and Biochemical Engineering, Graduate School of Engineering, Shizuoka University
Sumita, Kensuke Department of Medical Bioengineering, Okayama University
Watanabe, Kazunori Department of Medical Bioengineering, Okayama University
Okazaki, Shigetoshi Department of Medical Spectroscopy, Hamamatsu University School of Medicine
Abstract
In many drug delivery strategies, an inefficient transfer of macromolecules such as proteins and nucleic acids to the cytosol often occurs because of their endosomal entrapment. One of the methods to overcome this problem is photochemical internalization, which is achieved using a photosensitizer and light to facilitate the endosomal escape of the macromolecule. In this study, we examined the molecular mechanism of photochemical internalization of cell penetrating peptide-cargo (macromolecule)-photosensitizer conjugates. We measured the photophysical properties of eight dyes (photosensitizer candidates) and determined the respective endosomal escape efficiencies using these dyes. Correlation plots between these factors indicated that the photogenerated (1)O2 molecules from photosensitizers were highly related to the endosomal escape efficiencies. The contribution of (1)O2 was confirmed using (1)O2 quenchers. In addition, time-lapse fluorescence imaging showed that the photoinduced endosomal escape occurred at a few seconds to a few minutes after irradiation (much longer than (1)O2 lifetime), and that the pH increased in the endosome prior to the endosomal escape of the macromolecule.
Note
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Published Date
2015-12
Publication Title
Scientific Reports
Volume
volume5
Start Page
18577
End Page
18577
ISSN
2045-2322
Content Type
Journal Article
Official Url
http://www.nature.com/srep
language
英語
Copyright Holders
(c) Authors
File Version
publisher
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT