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ID 31685
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Author
Kondo, Junichi
Shimomura, Hiroyuki
Fujioka, Shin-ichi
Iwasaki, Yoshiaki ORCID Kaken ID researchmap
Takagi, Shinjiro
Ohnishi, Yasuhiro
Tsuji, Hideyuki
Sakaguchi, Kosaku
Tsuji, Takao
Abstract

The preS2 region of the hepatitis B virus (HBV) has been reported to have human polymerized albumin receptor (PAR) activity, which correlates with viral replication. Here, we studied the genomic sequence of the preS region from rare patients lacking PAR activity, despite active viral replication. PAR and DNA polymerase activity was identified in 178 HBe antigen-positive HBV carriers, and a significant correlation between 2 markers was shown, except in 2 hepatitis patients lacking PAR activity. Nucleotide sequences of the preS region of HBV from both patients were examined by direct sequencing of PCR products. In one patient, a 45-base deletion was found to overlap half of the putative polymerized human albumin binding site in the preS2 region. In the other patient, a point mutation at the first nucleotide of the start codon of the preS2 region of HBV was found. There was no such genomic change in the 3 control HBV sequences. These results indicate that the preS2 region is necessary for binding of polymerized human albumin, and this is the first report of naturally existing mutant virus with no or low PAR activity.

Keywords
hepatitis B virus
preS region
polymerized albumin receptor
genetic mutation
genetic deletion
Amo Type
Article
Published Date
2002-08
Publication Title
Acta Medica Okayama
Volume
volume56
Issue
issue4
Publisher
Okayama University Medical School
Start Page
193
End Page
198
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT