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ID 17390
Eprint ID
17390
FullText URL
Thumnail 90_841.pdf 411 KB
Title Alternative
Studies on the cornin extracted from liver 2. Purification of the rat liver microsomal cornin and its chemical properties
Author
Doi, Akitaka
Abstract
A curude cornin extracted from rat liver microsomes and the chemical properties of the purified cornin (MF1) were investigated. 1. Crude rat liver microsomal cornin (RLMC) inhibits the growth of L-929 cells. MF1 is purified about 4 fold from RLMC by gel filtration through Sephadex G-50 column. 2. The concentration of MFI that inhibits the growth of L cells to 50% is 270 μg/ml and this specific activity is the highest of the cornin fractions reported up to date. 3. The content of acidic amino acids is more than 23% but that of histidine and sulfurcontaining amino acids is very low in MFl. 4. MFl shows the maximum ultraviolet absorption at 276nm and its main substance is a kind of protein, and its inhibitory effect on the cell growth diminishes by protease treatment. 5. MFl is a single peak both by polyacrylamide gel electrophoresis and by thin layer gel filtration and the molecular weight is calculated about 26,000. 6. MFl is separated in two bands by SDS-polyacrilamide gel electrophoresis and the molecular weight is estimated to be 11,000 and 15,000 respectively.
Published Date
1978-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume90
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
841
End Page
849
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/90/7-8/90_7-8_841/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma