JaLCDOI 10.18926/AMO/30764
FullText URL fulltext.pdf
Author Ino, Hideo| Hayashi, Kazuhiko| Yanai, Hiroyuki| Teramoto, Norihiro| Koirala, Tirtha Raj| Chen, Hong-Li| Oka, Takashi| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaastu|
Abstract <p>A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.</p>
Keywords Epstein-Barr virus HVMF 1 lymphoma ?monkey cell line PCR
Amo Type Article
Published Date 1997-08
Publication Title Acta Medica Okayama
Volume volume51
Issue issue4
Publisher Okayama University Medical School
Start Page 207
End Page 212
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9284968
Web of Science KeyUT A1997XU03200004