FullText URL elife_7_30246.pdf
Author Takeda, Tetsuya| Kozai, Toshiya| Yang, Huiran| Ishikuro, Daiki| Seyama, Kaho| Kumagai, Yusuke| Abe, Tadashi| Yamada, Hiroshi| Uchihashi, Takayuki| Ando, Toshio| Takei, Kohji|
Keywords EM HS-AFM amphiphysin biophysics cell biology dynamin human in vitro reconstitution membrane remodeling structural biology
Published Date 2018-01
Publication Title eLife
Volume volume7
Publisher eLife Sciences Publications
Start Page e30246
ISSN 2050-084X
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
File Version publisher
PubMed ID 29357276
DOI 10.7554/eLife.30246
Web of Sience KeyUT 000423036500001
Related Url isVersionOf https://doi.org/10.7554/eLife.30246
FullText URL ijo_49_3_877.pdf
Author Yamada, Hiroshi| Takeda, Tetsuya| Michiue, Hiroyuki| Abe, Tadashi| Takei, Kohji|
Published Date 2016-06-30
Publication Title International Journal of Oncology
Volume volume49
Issue issue3
Publisher Spandidos
Start Page 877
End Page 886
ISSN 1019-6439
NCID AA10992511
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version publisher
PubMed ID 27572123
DOI 10.3892/ijo.2016.3592
Web of Sience KeyUT 000382447300003
Related Url isVersionOf https://doi.org/10.3892/ijo.2016.3592
JaLCDOI 10.18926/AMO/32823
FullText URL fulltext.pdf
Author Nakanishi, Akira| Kinuta, Keiko| Abe, Tadashi| Araki, Kenta| Yoshida, Yumi| Liang, Shuang| Li, Shun-Ai| Takei, Kohji| Kinuta, Masahiro|
Abstract <p>Administration of phenylhydrazine to rabbits resulted in the denaturation of hemoglobins in erythrocytes, causing the formation of intracellular precipitates known as Heinz bodies, severe hemolytic anemia, and reticulocytosis. To elucidate the molecular mechanism of the destabilization, we allowed human oxyhemoglobins to react aerobically with phenylhydrazine. After treatment with acetic acid/HCl and H2SO4/methanol, the chloroform extract contained blue-green pigments of major products accompanied by different minor products. Each product was isolated by column chromatography. By fast-atom-bombardment mass spectrometry (FAB-MS) and proton nuclear magnetic resonance (1H-NMR) spectrometry, dimethyl esters of N-phenylprotoporphyrin IX and meso, N-diphenylprotoporphyrin IX were determined. Other major products also were determined to be dimethyl esters of triphenyl-and tetraphenyl-substituted protoporphyrins by FAB-MS. The formation of meso, N-diphenylprotoporphyrin indicated that the addition of a phenyl radical to the meso-carbon atom of the protoporphyrin ring occurred. Triphenyl and tetraphenyl adducts also indicated the formation of phenyl radicals in the aerobic reaction of phenylhydrazine with oxyhemoglobins. From these results, we suggest that the formation of phenyl radicals and the replacement of heme with phenyl-substituted protoporphyrins cause the destabilization of hemoglobins to induce Heinz bodies and hemolytic anemia with phenylhydrazine.</p>
Keywords phenylhydrazine hemoglobin protoporphyrin fast-atom-bombardment mass spectrometry(FAB-MS) proton nuclear magnetic resonance(H-NMR)spectrometry
Amo Type Article
Published Date 2003-10
Publication Title Acta Medica Okayama
Volume volume57
Issue issue5
Publisher Okayama University Medical School
Start Page 249
End Page 256
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 14679403
Web of Sience KeyUT 000186186000006
JaLCDOI 10.18926/AMO/32307
FullText URL fulltext.pdf
Author Wrobel, Maria| Ubuka, Toshihiko| Yao, Wen-Bin| Abe, Tadashi|
Abstract <p>The effect of exogenous thyroxine (T4) administration on the activity of rhodanese, cystathionase, and 3-mercaptopyruvate sulfurtransferase (MPST) in the mitochondrial and cytosolic fractions of mouse liver was investigated. Three groups of mice were treated for 6 consecutive days with subcutaneous injections of T4 (50 micrograms, 100 micrograms, and 250 micrograms per 100 g of body wt, respectively). The other 3 groups were given 100 micrograms of T4 per 100 g of body wt for 1, 2, or 3 days. The dose of 100 micrograms T4 per 100 g of body wt given for 6 days exerted the strongest effect on the activity of all of the investigated enzymes. In comparison to the control, rhodanese activity diminished in the mitochondrial fraction by 40% (P &#60; 0.05), cystathionase activity diminished in the cytosolic fraction by 15% (P &#60; 0.05), and MPST activity in the mitochondrial fraction was reduced by 34% (P &#60; 0.05), whereas cytosolic MPST activity was unaltered. Simultaneously, in the liver homogenate, elevated levels of ATP and sulfate were observed after 6 days of T4 administration. Thus, the present results seem to suggest that in the mouse liver, after 6 days of administration of 100 micrograms T4 per 100 g of body wt, the desulfuration metabolism of L-cysteine is diminished, which is probably accompanied by an increase in oxidative L-cysteine metabolism. The dose of 100 micrograms per 100 g of body wt administered for a shorter period, and the use of a lower dosage (50 micrograms T4 per 100 g of body wt) for 6 days had a stimulatory effect upon MPST activity level, and an increased level of sulfane sulfur was observed.</p>
Keywords thyroxine rhodanese 3-mercaptopyruvate sulfurtransferase cystathionase sulfane sulful
Amo Type Article
Published Date 2000-02
Publication Title Acta Medica Okayama
Volume volume54
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 14
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10709617
Web of Sience KeyUT 000085526000002
JaLCDOI 10.18926/AMO/31647
FullText URL fulltext.pdf
Author Kurozumi, Yoshiatsu| Abe, Tadashi| Yao, Wen-Bin| Ubuka, Toshihiko|
Abstract <p>Experimental beta-alaninuria was induced in rats by injection of (aminooxy)acetate (AOA), a potent inhibitor of aminotransferases, in order to elucidate the pathogenesis of hyper-beta-alaninemia. A 27-fold increase of beta-alanine (BALA) excretion was induced by subcutaneous injection of 1 5 mg of AOA per kg of body weight. A 13-fold and a 9-fold increase of beta-aminoisobutyric acid (BAIBA) and gamma-aminobutyric acid (GABA), respectively, were also induced simultaneously by the AOA injection. Identification of BALA and BAIBA isolated from the rat urine was performed by chromatographic and mass spectrometric analyses. The effects of AOA injection on the tissue levels of these amino acids were also studied. Contents of BALA in the liver and kidney and GABA in the brain increased significantly in response to AOA injection. The present study indicates that BALA transaminase is involved in hyper-beta-alaninemia.</p>
Keywords beta-alanine beta-aminoisobutyric acid ganma-amlnobutyric-acid (aminooxy)acetate aminotransferase
Amo Type Article
Published Date 1999-02
Publication Title Acta Medica Okayama
Volume volume53
Issue issue1
Publisher Okayama University Medical School
Start Page 13
End Page 18
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
Web of Sience KeyUT 000078897700003
JaLCDOI 10.18926/AMO/31315
FullText URL fulltext.pdf
Author Yukihiro, Keishi| Tomozawa, Masaru| Abe, Tadashi| Yao, Wen-Bin| Ohta, Jun| Ubuka, Toshihiko|
Abstract <p>Sulfate and taurine are the main metabolites of L-cysteine in mammals and are excreted in the urine. The effect of a high protein diet on the ratio of sulfate to taurine excretion was studied in rats using synthetic 25% (standard protein diet group, group A) and 40% (high protein diet group, group B) casein diets. Average taurine and sulfate excretions (mumol/kg of body weight per day) were 280.4 +/- 93.8 and 943.2 +/- 144.8 in group A and 553.4 +/- 124.5 and 2675.0 +/- 390.9 in group B, respectively. Thus, the average taurine/sulfate ratio in group A was 0.30 +/- 0.08. By a single administration of 5 mmol of L-cysteine/kg of body weight in group A, the average taurine and sulfate excretions increased to 1127.5 +/- 120.2 and 4043.0 +/- 305.6, respectively, but the taurine/sulfate ratio changed only slightly (0.28). The average taurine/sulfate ratio in group B was 0.22 +/- 0.07, a significantly lower ratio than that in group A, which means that daily intake of a high protein diet resulted in more sulfate excretion. The taurine/sulfate ratio in group B was affected only slightly (0.19) by the cysteine administration as well. These results suggest that the ratio of taurine and sulfate production was determined by dietary protein content and that the increase in sulfate production is larger than that of taurine production when the intake of dietary protein is increased.</p>
Keywords high protein diet sulfate taurine cysteine metabolism
Amo Type Article
Published Date 1998-04
Publication Title Acta Medica Okayama
Volume volume52
Issue issue2
Publisher Okayama University Medical School
Start Page 71
End Page 75
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9588221
Web of Sience KeyUT 000073363000001
JaLCDOI 10.18926/AMO/31312
FullText URL fulltext.pdf
Author Tomozawa, Masaru| Yukihiro, Keishi| Yao, Wen-Bin| Abe, Tadashi| Ohta, Jun| Ubuka, Toshihiko|
Abstract <p>The effects of a low protein diet on the excretion of sulfate and taurine, major metabolites of L-cysteine in mammals, were studied in rats fed with synthetic 10% (group A) and 25% (group B) casein diets. The average excretions of total taurine (taurine plus hypotaurine) and total sulfate (free plus ester sulfate) (mumol/kg of body weight per day after the adaptation to the synthetic diet) in group A were 14.2 +/- 13.4 and 122.3 +/- 39.6, respectively, which were very low compared with 280.4 +/- 93.8 and 943.2 +/- 144.8, respectively, in group B. The taurine/sulfate ratio in group A was 0.12 +/- 0.11, which was significantly lower than that (0.30 +/- 0.08) in group B. A single intraperitoneal injection of 5 mmol of L-cysteine per kg of body weight in group A resulted in an increase in average taurine and sulfate excretion to 693.4 +/- 195.6 and 2440.6 +/- 270.0, respectively, and thus the average taurine/sulfate ratio increased to 0.29. These increases were transient and low taurine excretion resumed again 24 h after the L-cysteine administration. L-Cysteine injection in group B resulted in a similar increase in taurine and sulfate excretion, but the ratio changed only slightly (0.28). The present results suggest that in vivo production of taurine is reduced preferentially over sulfate production when sulfur amino acid supply is limited. </p>
Keywords low protein diet taurine sulfate crstein metabolism
Amo Type Article
Published Date 1998-04
Publication Title Acta Medica Okayama
Volume volume52
Issue issue2
Publisher Okayama University Medical School
Start Page 77
End Page 81
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9588222
Web of Sience KeyUT 000073363000002
JaLCDOI 10.18926/AMO/30954
FullText URL fulltext.pdf
Author Nakanishi, Akira| Abe, Tadashi| Watanabe, Masami| Takei, Kohji| Yamada, Hiroshi|
Abstract <p>Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound <i>in vitro</i>. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.</p>
Keywords dynamin amphiphysin phagocytosis testis
Amo Type Original Article
Published Date 2008-12
Publication Title Acta Medica Okayama
Volume volume62
Issue issue6
Publisher Okayama University Medical School
Start Page 385
End Page 391
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
Web of Sience KeyUT 000262025000005
JaLCDOI 10.18926/AMO/30413
FullText URL fulltext.pdf
Author Zhao, Yuan-Qing| Kinuta, Masahiro| Abe, Tadashi| Yao, Wen-Bin| Ubuka, Toshihiko|
Abstract <p>The effects of intraperitoneal administration of 2-(4-carboxy-D-gluco-tetrahydroxybutyl)thiazolidine-4-carboxylic acid (CGUA), a cysteine derivative conjugated with glucuronic acid, on total glutathione and total cysteine contents in rat tissues were investigated. Total glutathione (GSH and GSSG) and total cysteine (cysteine and cystine) were determined by a new method consisting of preparation of S-carboxymethylglutathione (CMSG) and S-carboxymethylcysteine (CMC), respectively, and subsequent analyses with an amino acid analyzer. CGUA was determined by a coloration method employing an acidic ninhydrin reagent. Total cysteine contents in liver, kidney and plasma rapidly increased to 2.3, 2.7 and 6.5 times the levels of the controls, respectively, after CGUA administration at a dose of 5 mmol/kg of body weight. Total glutathione content did not change significantly in the liver or blood except for the kidney with a significant increase during the first 1-h period after administration. CGUA content increased markedly in these tissues, especially in the kidney, and 30% of administered CGUA was excreted in urine within 2h. These results indicate that CGUA is converted into cysteine in vivo, suggesting the usefulness of this compound for protection of the kidney and the liver.</p>
Keywords cysteine glutathione S-carboxymethylglutathione S-carboxymethylcysteine cysteineglucuronic acied
Amo Type Article
Published Date 1995-02
Publication Title Acta Medica Okayama
Volume volume49
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 42
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7762408
Web of Sience KeyUT A1995QK32500006