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ID 13759
Eprint ID
13759
FullText URL
Thumnail 106_717.pdf 1.83 MB
Title Alternative
Cloning and structural analysis of mouse Apex gene encoding the major apurinic/apyrimidinic endonuclease
Author
Nagao, Kazutaka
Abstract
The APEX nuclease is a mammalian multifunctional repair enzyme having 5' apurinic/apyrimidinic (AP) endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3' phosphatase activities. The mouse Apex gene for the enzyme, was isolated from a mouse leukocyte genomic library by plaque hybridization with the mouse Apex cDNA as a probe. The nucleotide sequence of the Apex gene and its 5'-and 3'-flanking regions were determined. With reference to the published Apex cDNA sequence, the mouse Apex gene can be divided into five exons and four introns with a total length of about 2.6 kb. The boundaries between exon and intron follow the GT/AG rule. The translation initiation and termination sites are located in exons Ⅱ and Ⅴ, respectively. A part of the 5' flanking region belongs to a CpG island, which extends to intron Ⅱ. The CpG island is thought to be a putative transcription regulatory region of the Apex gene, a housekeeping gene.
Keywords
APEX ヌクレアーゼ
AP エンドヌクレアーゼ
DNA 修復酵素
Apex 遺伝子(マウス)
CpG island
Note
原著
Published Date
1994-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
717
End Page
730
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/106/7-8/106_7-8_717/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma