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ID 17754
Eprint ID
17754
FullText URL
Thumnail 86_299.pdf 4.17 MB
Title Alternative
CONTRIBUTION OF CELL SURFACE TO SALT TOLERANCE OF STAPHYLOCOCCUS AUREUS -STUDIES BY FREEZE-ETCHING METHOD-
Author
Tomochika, Ken-ichi
Sasai, Katsuko
Kanemasa, Yasuhiro
Abstract
The role of cell surface in a haloresistant mechanism of S. aureus was analyzed by free-ze-etching method. Our comparative studies were carried out on S. aureus cultivated in 0.5% NaCl containing medium (Normal-Staph) and in 10% NaCl containing medium (10% -Staph), as they were or were treated with L-11 enzyme (cell wall lytic enzyme obtained from Flabobacterium sp.). 1. As shown in photographs of non-treated and enzyme-treated cells, both the cell size and cell wall thickness of 10% -Staph increased to about 1.5 times Normal-Staph. 2. When Normal-Staph was exposed to L-11 enzyme, there occurred a gradual decrease in the OD. In the case of 10% -Staph, the OD showed a down-slope with a shoulder which rose in OD once at about 10 minutes after incubation. 3. The surface pattern of plasma membrane of Normal-Staph appeared to be made up of closely arranged spherical subunits. But in the case of 10% -Staph, the spherical subunits were indistinct and appeared to be sparsely located.
Published Date
1974-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume86
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
299
End Page
307
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/86/7-8/86_7-8_299/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma