Author Watanabe, Akiharu| Izumi, Masaki| Mizutani, Shigeki| Fujii, Toshitake| Watanabe, Masahiro| Higashi, Toshihiro| Yoshida, Hideki| Endo, Hiroshi| Nagashima, Hideo|
Published Date 1980-04-30
Publication Title 岡山医学会雑誌
Volume volume92
Issue issue3-4
Content Type Journal Article
JaLCDOI 10.18926/15291
Title Alternative Senior high school students' recognition and interest in allied medical education of college
FullText URL 007_2_113_120.pdf
Author Ohta, Takeo| Shimoishi, Yasuaki| Kageyama, Jingo| Shibuya, Koichi| Tohge, Hiroko| Endo, Hiroshi|
Abstract 1996年7~9月にかけて,岡山県周辺の10県下高校普通科3年在学中の学生に志望進路,志望分野とともに大学,短期大学における医療技術教育への認識と関心についてアンケート調査を行った。サンプリングによって依頼した127校のうち回答が得られたのは52校(回収率40.9%)で,得られた回答数は合計1,998人(男子891人,女子1,077人,不明30人)であった。これらの分析から,これらの地域では進学志望者の率は高く,特に男子に著名で,男女ともに理系への志望が多いことを認めた。医療技術系大学の認識は看護学科を除いて低く,関心も特に男子で低かった。4年制大学の進学を志望する学生にとっては, 3年制の多い医療技術系短期大学には関心が寄せがたく, 4年制へ移行した場合には関心も志望も高まる事が認められた。将来この分野を担う人材の確保には現在の医療短大の4年制化や医療技術職の待遇改善が重要である と結論づけた。
Abstract Alternative A survey was carried out by senior high school students in Okayama and nine surrounding prefectures from July through September, 1996. Data obtained from 1998 twelfth-grade students studying general courses at 52 schools were computed. Eighty four percent of the 891 male students and 54.2% of the 1,077 female students wished to go on to senior college. Sixtyseven percent of the male students wished to enter science college courses, while only 25.8% of female students did. The rate of students who knew about the course of allied medical science in senior or junior colleges was 63.8% for male students and 82.5% for female students. The nursing course was popular among both sexes, but other courses such as radiological technology, medical technology, physical therapy and occupational therapy were not well known, especially among male students. It was thought that students wishing to go on to the senior college were not interested in those areas which were usually taught in three year courses at junior colleges or special (vocational) schools. Accordingly, the data showed that their interests in the allied medical course would be increased by the shifting it to a four year course. To attract senior high school students who are both intelligent and talented in the medical and health field, the authors concluded that the further improvement of educational course for allied medicine, especially such as shifting to a four year course, and the bettering of position of comedical workers through it are needed.
Keywords 医療技術教育 (the allied medical education) 高校生 (senior high school students) 短期大学 (junior college) 大学 (college) 進学 (high school students wishing to go on to college)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-01-31
Volume volume7
Issue issue2
Start Page 113
End Page 120
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002307378
JaLCDOI 10.18926/15289
Title Alternative 変形性膝関節炎患者膝関節液中の細胞外マトリックス成分と可溶性VCAM-1
FullText URL 007_2_099_103.pdf
Author Tohge, Hiroko| Ichimura, Mitsuko| Sakiyama, Junko| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi|
Abstract It is generally accepted that the number of patients with osteoarthritis (OA) of the knee must increase in the future because of the increase of the aged. The pathogenic mechanisms of OA haven't been elucidated yet, but it is supposed that mediate pannus may cause the factors for irreversible cartilage damage in the inflammatory process. Therefore it is very important to find and predict OA at the early stage. Now there were many indices to determine joint damage with OA, for example, cytokines including IL, TNF, TGF, matrix metalloproteinase (MMPs), tissue inhibitor of metalloproteinase (TIMPs), proteoglycan (PG), hyaluronate (HA), super oxide, adhesion molecules including, ICAM-1, VCAM-1 and PECAM. In this study, we measured that the soluble VCAM-1 (sVCAM-1), soluble collagen (sCOLL), heparansulfate proteoglycan (HSPG), uronic acis and CRP in synovial fluids (SF) with OA. A positive correlation between the level of sVCAM-1 and sCOLL (ρ = 0.652) was noted. However there was no factor showing a correlation with CRP. It was suggested that the levels of sVCAM-1 and sCOLL in the synovia fluid were active inflammation indices of knee damage, as the different aspects from CRP. The level of HSPG also showed a unique trend because HSPG on the cell surface was quickly digested endocytosis, so that it was not suitable for the inflammatory index in synovial fluid with OA.
Abstract Alternative 変形性膝関節炎(OA)患者は,高齢化社会の到来とともに今後ますます増加するものと考えられる。OAの発症メカニズムについては,関節軟骨の破壊・修復やパンヌス形成に関わる多くの関連因子(サイトカイン,マトリックス分解酵素,分解酵素抑制因子,プロテオグリカン,活性酸素,細胞接着因子など)について,多面的に研究が進んでいる。しかし,膝関節の炎症初期の病態のメカニズムについては,なお詳細に解明されておらず,関節炎初期の炎症指標の検索が今後さらに必要と考えられる。本研究で私たちは, OA患者膝関節液中の細胞外マトリックス構成成分であるコラーゲン,へパラン硫酸プロテオグリカン,ウロン酸および炎症の最も初期に血管内皮細胞に出現するVCAM-1等の可溶化成分濃度を測定した。患者膝関節液(SF)中の可溶性コラーゲン量と可溶性VCAM-1(sVCAM-1)量とは有意な正の相関関係が(ρ=0.652)観察された。しかし,ヘパラン硫酸プロテオグリカン(HSPG)と可溶性コラーゲン(sCOLL)値間には有意な相関関係は確認されなかった。また,一般に炎症の指標として用いられるCRP値とも比戟検討したが,CRP値と他の測定項目値とは一定の相関関係は存在しなかった。以上の結果より,OA患者のSF中の炎症マーカ-として,急性炎症時には従来の炎症指標蛋白質であるCRP値が有力であるというSipe JDの報告から推測するとsCOLLとsVCAM-1はCRP値と一定の相関を示さないことより,CRPの動態とは異なった炎症指標としての有用性が考えられる。しかし,HSPGはエンドサイトシスにより速やかに代謝されるためSF中への解離が少なく,炎症指標としての可能性は低いと考えられる。
Keywords 変形性膝関節炎 (Osteoarthritis) 細胞外マトリックス (Extracellular matrix) 可溶性VCAM-1 (Soluble VCAM-1) 可溶性コラーゲン (Soluble Collagen) 可溶性ヘパラン硫酸プロテオグリカン (Soluble HSPG)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-01-31
Volume volume7
Issue issue2
Start Page 99
End Page 103
ISSN 0917-4494
language 英語
File Version publisher
NAID 120002307341
JaLCDOI 10.18926/15283
Title Alternative Views concerning nursing and nursing course of senior high school guidance teachers
FullText URL 008_1_043_048.pdf
Author Ohta, Takeo| Shimoishi, Yasuaki| Kageyama, Jingo| Shibuya, Koichi| Tohge, Hiroko| Endo, Hiroshi|
Abstract 1993年に岡山県で行った先の研究で,高校生の看護課程の選択にあたって,進路指導を行っている教師の役割が大きい事を報告した。今回は3年経った1996年に,同じ郵送調査を岡山県および周辺10県の普通科高校617校より無作為に選んだ127校において実施した。64校より得られた回答を要約すると以下の通りであった。1.教師が挙げた看護婦不足の理由はまず「勤務時間が厳しい(85.9%)」,「仕事の内容が厳しい(53.1%)」といった仕事のきつさで,次いで「仕事の割には社会的評価が低い(32.8%)」であった。2.教師が学生に肯定的イメージとして看護課程を勧める理由は,「まず専門職であること(73.4%)」,次いで「人に貢献できる喜び(53.1%)」であった。これらの結果は前回の結果と比較して統計的な有意差は認めなかった。3.近年進められている3年制看護課程(準学士課程)から4年制課程(学士課程)への移行に関して,教師はこれらは学生の関心を呼ぶとし(79.8%),看護課程へ進学する者は増加するであろうとしている(57.8%)。またこれまでよりこの課程への進学を教師は勧め易くなるとしている(56.3%)。これらの率は前回の結果と比較すると統計的に有意に高かった。
Abstract Alternative A previous study in Okayama prefecture in 1993 indicated the importance of high school teachers' influential rule on student's choice of nursing course. The same questionnaire survey was sent out by mail in 1996 to guidance teachers of 127 general high schools randomly selected from 617 schools in 10 prefectures, in and around Okayama. Data obtained from 64 teachers were analyzed and the results were as follows; 1. They suggested that shortage of nursing manpower was due to the harsh working conditions such as long and irregular hours (85.9%) and hard work (53.1 %), and also the low social esteem of nurse (32.8%). 2. The aspect of nursing noted by teachers were job specialty (73.4%) and devotion to people (53.l%). There were no statistical difference between these results and those of the previous study. 3. The teachers answered that the new four-year nuersing course (baccalaureate) currently being promoted from over the the present three-year course (associate degree) would be an incentive to students (79.8%) and might increase the number of students choosing to study nursing (57.8%). And they also noted that they would more readily recommend this course to students than before. These rates were significantly increased compared to the results of the previous report.
Keywords 看護課程 (nursing course) 看護 (nursing) 看護婦 高校教師 (high school teacher) 進路指導 high school student
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-09-10
Volume volume8
Issue issue1
Start Page 43
End Page 48
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002307697
JaLCDOI 10.18926/15268
Title Alternative ヒト血清リポタンパク質とシクロデキストリンのアガロースゲル内における相互作用
FullText URL 007_2_093_097.pdf
Author Ichimura, Mitsuko| Tohge, Hiroko| Sakiyama, Junko| Mori, Shuji| Ito, Sekiko| Nakata, Yasunari| Endo, Hiroshi|
Abstract Cyclodextrins (CDs) are cyclic oligosaccharides with low molecular weight. They have been known to bind to some serum lipoproteins and to form complexes. To elucidate whether each serum lipoprotein subclass could be separated by electrophoresis using CDs, we performed preliminary experiment, in which lipoprotein-CDs interaction was examined on electrophoresis with agarose gel. When the supporting agarose gel containing both α-CD and β-CD was prepared and was applied to isoelectric focusing for fractionating serum lipoproteins, apoB lipoproteins were found to be clearly separated into several fractions on this electrophoresis. This finding suggested that apoB lipoprotein may be detected as isolated form by arranging amounts of added CDs.
Abstract Alternative シクロデキストリンは低分子量の環状オリゴ糖で, リポタンパク質と複合体を形成する。シクロデキストリンを使用した電気泳動による血清リポタンパク質サブクラスの分離の可能性を明らかにするために, リポタンパク質とシクロデキストリンとの泳動用ゲル内での相互作用について検討をおこなった。その結果,α-CDとβ-CDの2種類のCDをアガロースゲルに添加し,このゲルを泳動用支持体として等電点電気泳動をおこなったところ,apoB含有リポタンパク質が数分画分離された。したがって,ゲル内へのシクロデキストリンの適正な添加条件が設定できるならば,apoB含有リポタンパク質サブクラスの分離が可能となることが示唆された。
Keywords 血清apoB含有リポタンパク質 (serum apoB lipoprotein) シクロデキストリン (cyclodextrin) 等電点電気泳動 (isoelectric focusing (IEF)) agarose gel
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1997-01-31
Volume volume7
Issue issue2
Start Page 93
End Page 97
ISSN 0917-4494
language 英語
File Version publisher
NAID 120002307426
JaLCDOI 10.18926/15259
Title Alternative Modified Method of Kluver-Barrera's Staining with Microwave Irradiation
FullText URL 008_2_107_112.pdf
Author Sakiyama, Junko| Fujikawa, Aya| Yamaji, Sachiko| Asano, Mayumi| Endo, Hiroshi|
Abstract クリューバー・バレラ(KB)染色は中枢神経系の白質における重要成分である髄鞘を選択的に染色する方法として日常的に広く使われている。しかし現在の所,ルクソール・ファスト青(LFB)染色後の0.05%炭酸リチウム及び70%アルコールによる分別時間が確定されていないので,分別の良否を経験的にしか判断せざるをえないという欠点がある。そこで,本研究において,マイクロウェーブ照射をLFB染色に応用することで,その後の分別について検討した結果,従来の0.05%炭酸リチウムでは良好な結果が得られなかったが分別液として,0.05%炭酸水素ナトリウムおよび70%アルコールを用いることで分別時間の確定が可能となった。
Abstract Alternative Kluver-Barrera's (KB) staining is the well-known and widely used method for staining the Schwann's sheath of the central nervous systems. On the contrary, the optimum time is not established yet, which incubated with 0.05% lithium carbonate and 70% alcohol after luxol fast blue staining. The modified method was studied that was stained with the microwave irradiation and used 0.05% sodium bicarbonate in stead of 0.05% lithium carbonate. This method was simple and useful to differentiate Schwann's sheath from other components.
Keywords クリューバー・バレラ染色 (Kluver- Barrera's staining) マイクロウェーブ照射 (Microwave irradiation) ルクソール・ファスト青液 (Luxol fast blue solution)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1998-01-31
Volume volume8
Issue issue2
Start Page 107
End Page 112
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002307709
JaLCDOI 10.18926/11924
Title Alternative Development of thermosensitive polymer-conjugated enzyme for repeated use.
FullText URL 005_087_090.pdf
Author Mori, Shuji| Endo, Hiroshi|
Abstract 温度感受性ポリマー誘導体を作製し、アルカリホスファターゼ(ALP)と結合させることによって、反復利用が可能なALPの開発を試みた。温度感受性ポリマー結合ALPは、反応混合液の温度を37℃以上に温度を上げることによって、簡単に他の成分から分離回収することができ、回収後も約80%の活性を保持していることが明かとなった。
Abstract Alternative For the purpose of repeated use of enzyme, alkaline phoshatase congugated with the reactive derivative of thermosensitive polymer was developed. This novel enzyme was precipitated immediately and recovered from reaction mixture, when the solution temperature was raised to 37℃. Recovered enzyme retained 80% activity compared to that of untreated one.
Keywords 温度感受性ポリマー (thermosensitive polymer) 反復利用 (repeated use) アルカリホスファターゼ (alkaline phosphatase)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 87
End Page 90
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313551
JaLCDOI 10.18926/11921
Title Alternative Comparative studies of proliferating cell nuclear antigen and bromodeoxyuridine staining pattern in rat regenerating liver cells
FullText URL 005_079_085.pdf
Author Sakiyama, Junko| Mori, Shuji| Ichimura, Mituko| Tohge, Hiroko| Okamoto, Motoi| Endo, Hiroshi|
Abstract 種々の条件下(10%緩衝ホルマリン・6時間、1%パラホルムアルデヒド・6時間、100%メタノール・4時間、70%エタノール・6時間、100%アセトン・2時間、カルノア液・4時間)で固定したラット再生肝組織に免疫組織染色を施し、Proliferating cell nuclear antigen (PCNA)の核内染色パターンについての検討を行った。その結果、100%メタノール及び70%エタノールで固定した場合には、明瞭な顆粒状の染色像が得られた。10%緩衝ホルマリン及び1%パラホルムアルデヒドの場合には、明瞭な顆粒状並びに弱いびまん性の染色像が得られた。S期に対する代表的なマーカーとして知られるBromodeoxyuridine (BrdU)の染色像との比較のもと、再生肝組織中のPCNA陽性細胞の数とBrdU陽性細胞の数を再生ピーク時の術後1日目で検討したところ、100%メタノール及び70%エタノールで固定した際の陽性細胞数が、最もBrdUの所見と一致した。しかし、10%緩衝ホルマリン、1%パラホルムアルデヒド、100%アセトン、カルノア液で固定した場合には、BrdUの所見と著しい解離が見られた。これらの知見は、PCNAを指標に細胞増殖を評価する上で、100%メタノール、70%エタノールでの固定が有用であることを示唆する。
Abstract Alternative Immunohistochemical staining pattern of proliferating cell nuclear antigen (PCNA) was examined in rat regenarating liver specimens fixed under the various conditions (10% buffered formalin for 6 hr, 1% paraformaldehyde for 6 hr, 100% methanol for 4 hr, 70% ethanol for 6 hr, 100% acetone for 2 hr and Carnoy solution for 4 hr). Most distinct granular staining pattern of PCNA was observed in fixation with 100% methanol or 70% ethanol. Specimens fixed with 10% buffered formalin or 1% paraformaldehyde showed both distinct granular and slight diffuse pattern of PCNA staining. Specimens fixed with 100% acetone showed only slight diffuse staining. Specimens fixed with Carnoy solution showed little staining. Number of PCNA positive cells in the specimens fixed with 100% methanol or 70% ethanol on 24 hours after hepatectomy coincided closely with those of BrdU positive cells, which is known to be a representative marker of cell proliferation. In contrast, dissociative result was obtained in specimens fixed with 10% buffered formalin, 1% paraformaldehyde, 100% acetone or Carnoy solution when numbers of PCNA positive cells were compared with those of BrdU. These findings suggest that tissue specimens fixed with 100% methanol or 70% ethanol is useful for evaluating the extent of cell proliferation.
Keywords PCNA BrdU 免疫組織染色 (immunohistochemical staining) 再生肝 (regenerating liver)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 79
End Page 85
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313417
JaLCDOI 10.18926/11918
Title Alternative リウマチ因子陽性ヒト血清中の免疫グロブリンGクラスリウマチ因子と免疫複合体ならびにCRPとの相関性
FullText URL 005_071_077.pdf
Author Tohge, Hiroko| Ichimura, Mitsuko| Sakiyama, Junko| Mori, Shuji| Nakata, Yasunari| Endo, Hiroshi|
Abstract We measured the concentration of three immunoglobulin classes of rheumatoid factors (Ig-RFs), C3d binding IgG immune complex (C3d-IC), C1q binding IgG immune complex (C1q-IC) and C-reactive protein (CRP) in 74 samples of human sera with high levels of RF (24.0~2350.0IU/ml). In sera with high levels of C3d-IC (>15.0μg/ml), there was a positive correlation between the levels of CRP and the IgG-RF, but there was no correlation between the levels of CRP and the immune complexes (C3d-IC and C1q-IC). And then, there was a positive correlation between the levels of CRP and IgG-RF or C3d-IC and IgG-RF when the levels of C1q-IC in patients sera were higher than 80.0μg/ml. However, there was no correlation between the levels of CRP and C1q-IC in these patients sera containing high levels of both C3d-IC and C1q-IC. These results indicated that the determination of C3d-IC, C1q-IC, IgG-RF and CRP in human sera containing RF denote different implications as inflammatory indexes on progression of rheumatoid arthritis and other autoimmune diseases.
Abstract Alternative 一般に慢性関節リウマチの診断ならびに活動性の一指標として、日常臨床検査では患者血清中のリウマチ因子の測定が実施されている。本論文ではRF検査依頼が有り、高RF値(24.0~2350.0IU/ml)を示した患者血清74検体について、免疫グロブリンクラス別RF値を測定するとともに自己IgGと補体との免疫複合体(IC)であるC1q-IgG・IC(C1q-IC)、C3d-IgG・IC(C3d-IC)ならびにCRP値を測定し、それらの測定値間の相関性について統計学的検討を行った。C3d-IC値が15.0μg/ml以上の高値を示す患者血清では、CRP値とIgGクラスのRF(IgG-RF)値間については正の相関結果がが得られた。また、C1q-IC値が80.0μg/ml以上の場合にもCRP値とIgG-RF値間およびC3d-IC値とIgG-RF値間では正の相関結果が得られた。しかし、C3d-ICならびにC1q-ICともに高値例の患者血清中のCRP値とC1q-IC値間には有意な相関は観察されなかった。以上の結果より、慢性関節リウマチの活動度を判定する上で、従来から炎症マーカーとしてCRP値が利用されているが、血清中に免疫複合体が高レベルに検出される患者については、炎症の指標としてCRP以外にもC1q-ICやC3d-ICおよびIgG-RF等を加えた総合的な判断が必要であることが示唆される。
Keywords self-associating IgG rheumatoid factor (自己IgG結合性リウマチ因子) C1q binding IgG immune complex (C1q結合IgG免疫複合体) C3d binding IgG immune complex (C3d結合IgG免疫複合体) C-reactive protein (C反応性タンパク質(CRP))
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 71
End Page 77
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313402
JaLCDOI 10.18926/11900
Title Alternative Faculties' ideas to the school syllabus
FullText URL 005_001_008.pdf
Author Ohta, Takeo| Shimoishi, Yasuaki| Azuma, Yoshiharu| Endo, Hiroshi| Okazaki, Yuka|
Abstract シラバスは、その内容も含めて、まだ教官にも、学生にもなじみのない言葉である。著者らは、1994年、初めてのシラバス(講義要覧)を刊行した。このシラバスの中では、1人もしくは数人の教官によって、1教科・1学期分の講義の概要が各1ページの中に記載され、その枠組みは、基本的事項以外に、抗議の目標、授業計画、授業計画、教科書、参考書、授業の進め方、評価の方法、教官のメッセージから成るものであった。このシラバス作成に参加した専任教官45人及び非常勤教官107人、計153人に対し、調査表によってシラバス作成に関する意見を求めた結果、117人(77.0%)の教官が解答を寄せた。その結果は次のようなものであった。1.今回の作成計画以前にシラバスについて認識があった者は44.5%であった。しかし、シラバスの今後の発行に関しては94.9%の者が賛成であった。今回の枠組みについてはほとんどの者が肯定的であった。2.学生による授業評価については、約半数(52.7%)が賛成であるが、時期尚早とする者が41.8%いた。
Abstract Alternative The term 'syllabus' and its concept are not familiar to university faculty members and students in Japan. Authors who belonged to School of Health Sciences Okayama University, published the first syllabus in 1994. In this syllabus, an outline of each course for a semester was shown by one or a few faculty members. The framework consisted of the mark of the subject, schedule, textbook, reference book, how to teach, how to grade and message to students, besides basic information. Forty-five full-time faculty members and 107 part-time faculty members were asked about their ideas for the syllabus through a questionnaire survey by mail and 119 faculty members (78.3%) responded to it. Only fifty-three faculty members (44.5%) had known the term 'syllabus' before the time when they started to talk about it or were asked to write it. But after their presenting syllabi, 104 faculty members (87.4%) agreeded to continue publishing and they were affirmative to framework as it was proposed this time. As for evaluation of subject by students, about half of faculties (52.7%) agreeded and 51.8% of them replied that were not mature enough for it yet.
Keywords シラバス (syllabus) 授業要覧 授業概要 教官の意見 (faculties' idea) evaluation by student
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1995-01-31
Volume volume5
Start Page 1
End Page 8
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313605
JaLCDOI 10.18926/11873
Title Alternative Clearance study of polyethylene glycol-modified cholesterol esterase.
FullText URL 004_053_055.pdf
Author Mori, Shuji| Nakata, Yasunari| Endo, Hiroshi|
Abstract The clearance of polyethylene glycol-modified cholesterol esterase was investigated spectrophotometrically, as compared with native one. PEG-modification provided the significant elongation in clearance time of cholesterol esterase after intravenous injection in mouse. This method was shown to be effective for creating new biomaterials.
Keywords cholesterol esterase polyethylene glycol modification in vivo clearance
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1994-01-31
Volume volume4
Start Page 53
End Page 55
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313810
JaLCDOI 10.18926/11871
Title Alternative IgG Heavy-Chain Subclass Typing of Human Serum M-Protein -Optimal condition for the separation and the detection with high resolution of agarose gel isoelectrofocusing-
FullText URL 004_047_051.pdf
Author Ichimura, Mitsuko| Tohge, Hiroko| Sakiyama, Junko| Nakata, Yasunari| Endo, Hiroshi|
Abstract IgG Heavy-Chain subclass typing of human serum M-protein were isoelectrofocussed (IEF) in agarose gel, and then the bands of IgG were detected with peroxidase-conjugated anti-human IgG1-4 monoclonal antibodies on the same isoelectrofocussed agarose gel plate. This IEF enzyme immunodetection patterns were composed of four to eight discrete bands (The range of pI was 6.0 to 9.0). These bands were dependent on immunofixationbands. It was very specific and clear to detect the subclass of IgG Type M-protein in human serum using this method.
Keywords IgG Subclass M-Protein Isoelectrofocusing Monoclonal Antibodies
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1994-01-31
Volume volume4
Start Page 47
End Page 51
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313808
JaLCDOI 10.18926/11847
Title Alternative The results of liver function test on workers handling ethanol and isopropanol solvents
FullText URL 003_093_097.pdf
Author Ohta, Takeo| Endo, Hiroshi| Mori, Shuji| Koda, Shigeki| Oze, Yutaka|
Abstract Liver function tests such as GOT, GPT, γ-GTP and ornithine carbamyl transferase (OCT) were done on workers handling alcoholic solvents. One was a group of molders working in a foundry where they sprayed ethanol solvent on the surface of a sandbox and burnt it. Another was a group of painters at an iron and steel works and they sprayed paint with isopropanol solvent on iron and steel plates. No statistical differences in the results of tests between the solvent workers and control groups were seen. High correlations were found between GOT/γ-GTP and OCT/γ-GTP. OCT of 4 workers who had drunk alcohol on the day before test showed a statisically high value compared to workers who had not drunk alcohol.
Keywords Ethanol Isopropanol Liver Function Test アルコール系溶剤 肝機能
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 93
End Page 97
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313962
JaLCDOI 10.18926/11818
Title Alternative Alcoholic Liver Disease
FullText URL 003_001_012.pdf
Author Endo, Hiroshi| Nakata, Yasunari| Okamoto, Motoi| Mori, Shuji| Ohta, Takeo|
Abstract Relationship between ethanol drinking and organs injury was reviewed and special emphasis was put on alcoholic liver disease. Consumption of alcoholic beverage expressed as ethanol per capita of adult in Japan increased 2.1 times in these 25 years and it is still increasing. Although the incidence of alcoholic liver disease in Japan also increased greatly during the above period, it seems likely that plateau level is coming because of genetically defined, unique type of alcohol metabolism in Japanese. Sex differences in susceptibility to alcohol were discussed. Among the six types of alcoholic liver disease, alcoholic liver fibrosis is relatively frequent in Japan. Mechanism of liver injury has been studied extensively. Alcohol itself is toxic but other factors such as dietary fat are also important. Biochemical and immunological markers of drinking were presented. As for the treatment, most patients especially in early stages of the disease well respond to alcohol withdrawal, but therapy of alcohol dependence in the background of the disease is very difficult requiring cooperative works of different kinds of specialists.
Keywords alcohol liver epidemiology genetic factors alcohol dependence
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1993-01-31
Volume volume3
Start Page 1
End Page 12
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313726
JaLCDOI 10.18926/11770
Title Alternative A study on students' idears about the school syllabus
FullText URL 007_1_029_033.pdf
Author Ohta, Takeo| Shimoishi, Yasuaki| Azuma, Yoshiharu| Ichimura, Mituko| Endo, Hiroshi| Okazaki, Yuka|
Abstract 1995年4月に初めての本学のシラバス(講義要覧)が発行され、学生に配布された。本報は約1年後の1996年2月の試験期間中にシラバスに対する学生の意見を問うために行われたアンケート調査の結果をまとめたものである。回答を寄せた学生は435人で、在籍学生の89.0%であった。よく利用したとする学生は18.2%、数回利用したとする学生は67.6%であった。学生の使用目的はテストの準備や履修科目の決定のためであった。以上のことから、現在のシラバスは学生が日常的により活用するためにはさらに教師が改善、工夫する必要があると思われた。
Abstract Alternative The first syllabus of the School was published and distributed to all of the students in April,1995. Ten months later during the term examination, an opportunity arouse to question the sudents about their idears for the syllubus. A survery of all of the students was carried out and 435 students (89.0%) responded to it. They generally utilized it well, especially to decide which subjects they will take, to find out about the textbooks that will be used, and how to prepare for the examination. However it was found not to be sufficient for their daily use. Teachers therefore must make a greater effort in giving information about their lecture.
Keywords シラバス (syllabus) 授業計画 講義要覧 学生の意見 (student) use
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 29
End Page 33
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002313997
JaLCDOI 10.18926/11760
Title Alternative Alteration of thermostable phosphatase activity after hydrophobic chromatography
FullText URL 007_1_017_021.pdf
Author Mori, Shuji| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi|
Abstract 耐熱性ホスファターゼを含んだBacillus stearothermophilus 粗酵素試料を、リソースIsoによる疎水性クロマトグラフィにかけ分離を行った。1.5M→0M 硫酸アンモニウムの直線逆濃度勾配によって溶出を行ったところ、ホスファターゼは不活性な形で溶出され、これは硫酸アンモニウムによる濃度依存的阻害に起因することが判明した。ホスタファーゼの反応混合液に種々の濃度の硫酸アンモニウムを添加したところ、0.15Mの硫酸アンモニウム存在下で約80%の阻害が認められた。加えて、この阻害作用は単に硫酸アンモニウムの添加によってpHが酸性側に傾くことによるものではないことも明らかとなった。
Abstract Alternative Thermostable phosphatase partially purified from thermophilic bacteria, Bacillus stearothermophilus, was chromatographed on Resource Iso hydrophobic resin. When linear reverse gradient elution with 1.5 M → 0 M ( NH(4))(2)SO(4) was performed, phosphatase was found to be eluted as latent form, which revealed dose-dependent inhibitory effect of (NH(4))(2)SO(4) on phosphatase. When various concentrations of (NH(4))(2)SO(4) were added into phosphatase reaction mixture, about 80% inhibition was observed in the presence of 0.15 M (NH(4))(2)SO(4). Acidification by adding (NH(4))(2)SO(4) was not responsible for this inhibition, because addition of (NH(4))(2)SO(4) solution which pH was previously adjusted to 9.0 showed same inhibitory effect.
Keywords 耐熱性ホスファターゼ (thermophilic phosphatase) 疎水性クロマトグラフィ (hydrophobic chromatography) 硫酸アンモニウム (ammonium sulfate) 濃度依存的阻害 (dose- dependent inhibition)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-08-30
Volume volume7
Issue issue1
Start Page 17
End Page 21
ISSN 0917-4494
language 日本語
File Version publisher
NAID 120002314041
JaLCDOI 10.18926/11759
Title Alternative 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について―
FullText URL 007_1_009_015.pdf
Author Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki|
Abstract Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues.
Abstract Alternative 10%緩衝ホルマリンに6ヶ月間浸漬していた剖検材料(肝臓癌)のパラフィン包埋後の組織切片を用いて、ホルマリンの固定作用により失活したPCNAの免疫反応性の回復にマイクロウェーブ(MW)及びオートクレーブ(AC)による熱処理とその時用いる処理溶液が、どのような影響を与えるかについて検討した。その結果、同等の免疫反応性を回復するのには、AC処理の方がMW照射より短時間でよいことが解った。しかし、MW照射による免疫反応性回復では、処理時間による影響がAC処理に比べて少なく、安定した結果が得られた。また、PCNAの染色所見については、AC処理の方が濃く染まる傾向が見られた。しかし、処理時間が長くなるとバックグラウンドの染色性が高くなる、核の周囲ににじみ現象が見られる等の所見があり判定に困難をきたした。一方、処理溶液についてはクエン酸(0.01M pH6.0)、チオシアン酸鉛飽和溶液、蒸留水について検討を行った結果、PCNAの回復には差は認められなかったが、バックグラウンドの染色については蒸留水が最も少なかった。以上の結果により、ホルマリン固定により失活したPCNAの免疫反応性を回復するには、MW照射の方がAC処理より処理時間に関係なく安定した染色性が得られ、なおかつバックグラウンドの染色性が少ない等の点で優れていることが示唆された。
Keywords PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定)
Publication Title 岡山大学医療技術短期大学部紀要
Published Date 1996-09-30
Volume volume7
Issue issue1
Start Page 9
End Page 15
ISSN 0917-4494
language 英語
File Version publisher
NAID 120002313519