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ID 18404
Eprint ID
18404
FullText URL
Thumnail 74_623.pdf 284 KB
Title Alternative
Studies on Glutamic Decarboxylase in the Brain I. A New Method for Determining Glutamic Decarboxylase Activity With the Use of (14)C-Glutamic Acid
Author
Kondo, Osamu
Abstract
1. A new method of determining the glutamic decarboxylase activity of the brain with the use of glutamic acid-1-(14)C has been devised by the author and its feasibility is discussed from various angles. 2. The erro in the determination of radioactivity of the experimental materials by this method is extremely small. 3. It is thought that there occurs no accumulation of (14)CO(2) during the determination of radioactivity by this method. 4. Since the self absorption by the material during the determination of its radioactivity is considerably high, a care should be excercised on a proper thickness of the material. 5. The incubation should be carried out under anaerobic condition in the experiment by this method. 6. The optimal pH for the system of this experiment is 6.6. 7. The amount of the substrate to be added to the system should be 5mg. 8. The incubation period of this experiment is set at two hours. 9. By applying this method on rabbit organs it has been proven that the activity of glutamic decarboxylase can be determined.
Published Date
1962-09-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume74
Issue
issue8-9
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
623
End Page
627
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/74/8-9/74_8-9_623/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma