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ID 58551
Author
Nakatsu, Yukiko Graduate School of Environmental and Life Science, Okayama University
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University Kaken ID
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University Kaken ID
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Abstract
Pseudomonas syringae pathovars are known to produce N-acyl-homoserine lactones (AHL) as quorum-sensing molecules. However, many isolates, including P. syringae pv. tomato DC3000 (PtoDC3000), do not produce them. In P. syringae, psyI, which encodes an AHL synthase, and psyR, which encodes the transcription factor PsyR required for activation of psyI, are convergently transcribed. In P. amygdali pv. tabaci 6605 (Pta6605), there is one nucleotide between the stop codons of both psyI and psyR. However, the canonical stop codon for psyI in PtoDC3000 was converted to the cysteine codon by one nucleotide deletion, and 23 additional amino acids extended it to a C-terminal end. This resulted in overlapping of the open reading frame (ORF) for psyI and psyR. On the other hand, stop codons in the psyR ORF of P. syringae 7 isolates, including pv. phaseolicola and pv. glycinea, were found. These results indicate that many pathovars of P. syringae have genetically lost AHL production ability by the mutation of their responsible genes. To examine whether PtoDC3000 modulates the gene expression profile in a population-dependent manner, we carried out microarray analysis using RNAs prepared from low- and high-density cells. We found the expressions of rsmX and rsmY remarkably activated in high-density cells. The activated expressions of rsmX and rsmY were confirmed by Northern blot hybridization, but these expressions were abolished in a ΔgacA mutant of Pta6605. These results indicate that regardless of the ability to produce AHL, P. syringae regulates expression of the small noncoding RNAs rsmX/Y by currently unknown quorum-sensing molecules.
Keywords
N-acyl-homoserine lactone
Gac two-component system
Quorum sensing
rsmX
rsmY
Pseudomonas syringae
Note
This fulltext is available in Sep. 2020
Published Date
2019-08
Publication Title
Microbiological Research
Volume
volume223-225
Publisher
Elsevier
Start Page
72
End Page
78
ISSN
09445013
NCID
AA11017780
Content Type
Journal Article
language
英語
File Version
author
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1016/j.micres.2019.04.004
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
15H04458