start-ver=1.4
cd-journal=joma
no-vol=67
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=11
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1955
dt-pub=19550131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of the Bone-Marrow Autolysate on Medullary Hematopoiesis Part I: Intra-abdominal injection and test of artificial circulation through the femur
kn-title=骨髓自家融解液の骨髓造血に及ぼす影響 第1編 腹腔内注射並に骨髄灌流実験
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author, as a link in the serial study on improvement of medullary hematopoiesis by so-called bone-marrow substance, tried to investigate both effects and functions of parenteral administration of rabbit's red bone-marrow autolysate (called simply as "bone-marrow extract" hereafter); so that, having injected its certain amount into normal rabbit's abdominal cavity, the author inspected its hourly effect on peripheral blood picture as well as on blood picture of V. nuturitica femoralis, and also took observations of certain changes that occurred to intramedullary circulation. As a result, the author discovered that: 1) the biphasic blood cell increase was induced in both cases of single and successive injection alike; 2) in its secondary blood cell increase, the increase was more remarkable in case of successive, than in case of single, injection; 3) above all, an increase of reticulocytes, a comparative increase of pseudoeosinocytes, and a left shift of leucocytes, were remarkably induced. Moreover, in case of the primary blood cell increase, an expansion of medullary nutric vessels, a marked blood cell increase in nutric veinal blood, and a comparative increase of pseudoeosinocytes --- i. e. a liberation of accumulated blood cells within bone-marrow --- were brought about; and further, the blood stream within bone-marrow under the two phases of blood cell increase was noticed to be remarkably stimulated, as compared with the case in normal rabbit. From the above results, it may roughly be said that, in case the bone-marrow extract should be intraabdominally injected, a biphasic blood cell increase occures in both cases of single and successive injection, the latter being more powerful in stimulating medullary hematopoiesis; and that the primary blood cell increase is caused by the mobilization as well as the liberation of accumulated blood cells which is due to the stimulation to blood stream within bone-marrow, while the secondary blood cell increase owes much to the hematopoietic function of bone-marrow which is originated in the continuance of stimulation to blood stream within bone-marrow.
en-copyright=
kn-copyright=

en-aut-name=FujiiMasatomi
en-aut-sei=Fujii
en-aut-mei=Masatomi
kn-aut-name=藤井昌富
kn-aut-sei=藤井
kn-aut-mei=昌富
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=67
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=27
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1955
dt-pub=19550131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of the Bone-Marrow Autolysate on Medullary Hematopoiesis Part II: Influence of bone-marrow autolysate under splenectomy, liver parenchymal disturbance or reticuloendotherial blockade
kn-title=骨髓自家融解液の骨髓造血に及ぼす影響 第2編 剔脾，肝障碍，網内糸填塞の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In the previous report, the author stated that there should happen a remarkably stimulated medullary hematopoiesis if the intra-abdominal injection of bone-marrow extract were successively rendered for a normal rabbit; this time, I conducted the following experiments in order to clarify whether these phenomena would activate indirectly through liver, spleen, or reticuloendotherial syetem, which are said to keep close relation with hematopoiesis, or, as Miyakawa maintained in this so-called "autohormone tbeory", functionate directly. First, having successively injected "bone-marrow extract" at 48 hours as well as 7 days after the splenectomy, the author examined its peripheral blood picture: this time, as was seen in the case of normal rabbit, biphasic blood cell increase was observed to take place, and an increase of reticulocytes number as well as a left shift of leucocytes were observed conspicuously, under the secondary blood cell increase. Consequently, it was verified that the same extract could stimulated the medullary hematopoiesis even under the influence of splenectomy. Next, by injecting likewise at 24 hours after the liver parenchymal disturbance, biphasic blood cell increase, an increase of reticulocytes number, and a left shift of leucocytes were noticed; moreover, even in case when the functions both of liver and spleen were removed at the same time, almost the same result as in the normal rabbit was obtained. In case the reticuloendotherial system was blocked with India-ink, even successive injection could not cause either increase of blood cell as well as left shift of leucocyte or improvement of intramedullary blood stream; that is to say, no medullary hematopoiesis was brought about. From the above results, the author concludes that the improvement of medullary hematopoiesis by successive intraabdominal injection of bone-marrow extract has no concern with either splenectomy or liver parenchymal disturbance, that the soundness of bone-marrow reticuloendotherial system should be indispensable requisite, and that this phenomenon might occur owing to the direct stimulation to bone-marrow parenchymal function.
en-copyright=
kn-copyright=

en-aut-name=FujiiMasatomi
en-aut-sei=Fujii
en-aut-mei=Masatomi
kn-aut-name=藤井昌富
kn-aut-sei=藤井
kn-aut-mei=昌富
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END

start-ver=1.4
cd-journal=joma
no-vol=67
cd-vols=
no-issue=1
article-no=
start-page=29
end-page=42
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1955
dt-pub=19550131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of the Bone-Marrow Autolysate on Medullary Hematopoiesis Part III: Experiment in adding the Autolysate to Bone-Marrow Tissue Culture
kn-title=骨髓自家融解液の骨髓造血に及ぼす影響 第3編 骨髄体外組織培養への添加実験
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author, about the improvement of bone-marrow hematopoiesis, presumed in Part I that the blood vessel function might be one cause; in Part II, concluded that the reticuloendotherial system should be sound a prior, and as was told in the so-called "autohormone theory" it might be due to the particular hormone-like function which is proper to that extract. Accordingly, in this part, in order to exactly verify whether the bone-marrow extract would be influenced directly to the medullary parenchymal Hematopoiesis or not, the author added that extract to bone-marrow tissue culture and inspected its growth rate, wandering velosity, and changes in quantity of hemoglobin as well as in number of blood cells. First, taking observations of the growth rate of bone-marrow tissue, and the wandering velocity of intramedullary pseudoeosinocytes, under culture in cover slips, it was known that the addition of extract in a proper density improves remarkably the growth of tissue, and that the main purport of its growing process roughly coincides with non-added case as well as with controls. Also, the wandering velocity as well as time of continuance of the pseudoeosinocytes, though it was remarkably inhibited when the extract proved very dense, almost receive no other influence, and no significant differences with the controls could be discovered; i.e., the author could perceive that the increase in tissue growth rate by adding the bone-marrow extract was no result of activation of cell wandering, but it owes to the stimulation of growth in parenchymal cells themselves. Moreover, taking observation of the effect of bone marrow extract on the increase of hemoglobin as well as red blood cell number, under the culture in fluid medium, it was clarified that a certain of extract in a proper density brought about a marked increase in the amount, proving the existence of direct function by the extract. All through 3 reports the author have vindicated the fact that the improvement of medullary hematopoiesis by parenteral administration of so-called bone-marrow substances, may be caused not only by the vessel function, but also by the function of bone-marrow substance which has power to advance hematopoietic function.
en-copyright=
kn-copyright=

en-aut-name=FujiiMasatomi
en-aut-sei=Fujii
en-aut-mei=Masatomi
kn-aut-name=藤井昌富
kn-aut-sei=藤井
kn-aut-mei=昌富
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部平木内科教室
END