JaLCDOI 10.18926/14927
Title Alternative サーモグラフィーによる体表面温度の測定 3.体表面温度の回復率と末消血流量との相関
FullText URL 070_036_042.pdf
Author Hosaki, Yasuhiro| Takata, Shingo| Mitsunobu, Fumihiro| Mifune, Takashi| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Harada, Seishi| Tanizaki, Yoshiro| Ochi, Koji| Tsuji, Takao|
Abstract The body surface peripheral circulation in 12 cases, including 9 patients with diabetes mellitus who were suffering coldness, numbness or pain in their feet, and 3 healthy volunteers was examined using Laser-Doppler blood flowmetry. At the same time, the body surface temperature was estimated by thermography. Thermographic results were analyzed quantitatively by calculating a recovery ratio as : Recovery ratio = [Total counts of thermography(Pixels) over temperature (T) after cold loading] ÷ [Initial counts over T before cold loading] x 100(%). The recovery ratio and the blood flow were correlated, r=0.68, p<O.01. The peripheral circulation of 16 patients with diabetes mellitus was observed at three different conditions including, l)placed at room temperature at 20℃for 15 min, 2) submerged and warmed for 5 min in a hot bath at 36℃(i.e. hot loading), and 3) submerged and cooled for 5 min in a water bath at 20℃ (i.e. cold loading). Three different baseline temperatures, 26℃, 27℃ and 28 ℃, were used in processing the thermographic results into pictures. The highest correlation (r=0.59, p=0.0002) was obtained under the condition of cold loading using a baseline temperature limitation of27℃.The difference ratio (%) of blood flow was calculated as the blood flow at cold loading divided by the blood flow at hot loading in these 16 patients. The difference ratio of the blood flow and the recovery ratio of thermography were correlated, r=0.46, p<O.OOO1. We found a strong correlation between the results of Laser-Doppler blood flowmetry and one of thermographic methods used to monitor peripheral circulation in patients with diabetes mellitus. Cold loading using a baseline temperature limitation of 27℃ were recommended for further examinations. Patients with low blood flow as well as with large differences in their peripheral circulation between cold loading and hot loading had severe coldness in their body surface temperature. We showed the usefulness of the results of thermography, when quantified by picture processing using computer software, in relation with the results of Laser-Doppler blood flowmetry.
Abstract Alternative 末梢神経障害を有する糖尿病患者の末梢循環障害の程度を数量的に検討する目的で、下肢に冷感ならびにしびれ感または疼痛を訴える糖尿病患者9症例と健常ボランティア3例の計12例(平均年齢59歳)についてサーモグラフィーを用いて体表面温度を測定した。更に,サーモグラフィーで得られた結果と末梢皮膚血流量をレーザードプラー血流計を用いて測定して得られた結果と比較した。サーモグラフィーによる測定で得られた結果は回復率として数量化して表示された。回復率の算出方法は回復率= [冷水負荷後の特定温度27℃以 上の体表面温度のサーモグラフィーのPixelの総数]÷ [冷水負荷前の特定温度27℃以上の体表面温度のサーモグラフィーのPixelの総数]×100%で求めた。レーザードプラ-血流計を用いて測定して得られた末梢血流量は左右それぞれ5カ所,計10カ所の測定値の平均で表示した。その結果,末梢皮膚温度の回復率と末梢皮膚の血流量との問には正の相関関係(r=0.68,p<0.01)が認められた。次に,末梢皮膚血流量について,室温20℃安静15分後,温水36℃浸水負荷10分後,冷水20℃浸水負荷30分後の異なる3条件について、またサーモグラフィーで得られた結果を,画像処理の過程で用いられた,26℃,27℃,28℃の3つの異なる特定温度との関連について検討を行なった。対象は,下肢に冷感ならびにしびれ感または疼痛を訴える糖尿病患者16症例(平均年齢69歳,平均HbAIC9.6%)について測定した。その結果,末梢皮膚 血流量は冷水20℃浸水負荷30分後に測定して得られた結果と,回復率は特定温度27℃で画像処理して得られた結果とが最も相関が高い(r=0.59,p=0.0002)ことが示された。`次に,相関が高い条件は,室温20℃安静15分後に血流量を測定した場合(r=0.483,p=0.0002)であった。そして温 水36℃浸水負荷10分後に測定して得られた結果とが最も相関関係が低い結果となった。更に,冷水20℃浸水負荷30分後に測定して得られた結果を温水36℃浸水負荷10分後に測定して得られた結果で割った比を%で表示したところ回復率とこの比との間には正の相関関係(r=0.46,p<0.0001)が認められた。このことから,温水36℃負荷時と,冷水20℃負荷時との差が大きい患者 において末梢皮膚温度の低下が著しいことが示された。これまで悲観血的に測定されてきたサーモグラフィーによる末梢循環の数量的評価の試みは,レーザー血流計による結果と組み合わせることで,両者の間に正の相関関係が示されたことにより,今後,数量化された客観的評価を可能にした。
Keywords サーモグラフィー (thermography) レーザードプラー血流計 (Laser-Doppler blood flowmetry) 糖尿病 (diabetes mellitus) 末梢循環 (peripheral circulation) 冷水負荷 (cold loading)
Publication Title 岡大三朝分院研究報告
Published Date 1999-12
Volume volume70
Start Page 36
End Page 42
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308240
JaLCDOI 10.18926/15088
Title Alternative サーモグラフィーによる体表面温度の測定
FullText URL 067_063_070.pdf
Author Hosaki, Yasuhiro| Nawa, Yuichiro| Takeuchi, Kazuaki| Tsugeno, Hirofumi| Ashida, Kozo| Yokota, Satoshi| Mitsunobu, Fumihiro| Mifune, Takashi| Tanizaki, Yoshiro| Ochi, Koji| Harada, Hideo| Ikeda, Satoru| Taketa, Kazuhisa|
Abstract Body Surface Temperature was observed by thermography. The surface temperature of a healthy person's lower limbs, after being cooled in a water bath, increased in parallel with time. Patients with Diabetes Mellitus had different patterns in the rate of increase of the surface temperature. After cold loading, one patient had a 99% recovery ratio of surface temperature, the same level as healthy volunteers (83%, and 88%), as calculated by a picture processing program with the computerized thermotracer. However, the recovery ratio of other patients was poorer, ranging from 56% to under 6% recovery. This measurement of elevation of body temperature is useful for the estimation of peripheral blood flow in patients with lower limbcirculation failure.
Abstract Alternative 健常人並びに糖尿病患者を対象にして下肢の体表面温度を測定した。測定にはサーモグラフィーを用い,得られた画像の数値化にはコンピュータを用いた画像処理システムを利用した。健常人を用いた実験では,20℃の水を用いた冷水負荷を5分間行うことにより,冷水負荷後,下肢の体表面温度は時間とともに上昇して30分後に良好な回復を得ることができた。画像処理により28℃以上の体表面温度を呈した下肢の面積を負荷前と比較したところ,回復率は,2名の健常人についてみるとそれぞれ83%,88%であった。27℃で画像処理を行った場合には回復率は93%となり過大評価される可能性があった。また,29℃で画像処理を行ったところに,逆に,64%となり過少評価される可能性があり,28℃が最も良い条件であった。この様な条件下で,糖尿病患者7名について同様に測定を行ったところ,1名は健常人と同じく99%の良好な回復率を呈した。しかし,他の1名は56%であり,残りの,5名は6%以下であった。この様に,糖尿病患者では,下肢の体表面温度の冷水負荷後の回復率に顕著な差を認めた。この差は,糖尿病患者における,末梢循環障害の程度を反映しているものと考えられた。この様にコンピュータを用いた画像処理システムの応用によりサーモグラフィーの画像は数値化することが出来,測定結果はより客観的に据えることが可能となった。画像処理されたサーモグラフィーは下肢の循環障害を持つ患者の末梢血流量の評価に有用な測定方法であると考えられた。
Keywords Thermography (サーモグラフィー) Diabetes Mellitus (糖尿病) Peripheral Circulation (末梢循環) Cold Loading (冷水負荷)
Publication Title 岡大三朝分院研究報告
Published Date 1996-11
Volume volume67
Start Page 63
End Page 70
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307566
Author Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Iwagaki, Naofumi| Fujii, Makoto| Takata, Shingo| Hamada, Masanori| Tanizaki, Yoshiro|
Published Date 2004-12-01
Publication Title 岡大三朝医療センター研究報告
Volume volume75
Content Type Departmental Bulletin Paper
Author Takata, Shingo| Ashida, Kozo| Hosaki, Yasuhiro| Hamada, Masanori| Iwagaki, Naofumi| Kikuchi, Hiroshi| Mitsunobu, Fumihiro|
Published Date 2008-03-01
Publication Title 岡大三朝医療センター研究報告
Volume volume76
Content Type Departmental Bulletin Paper
Author Ashida, Kozo| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Hamada, Masanori| Iwagaki, Naofumi| Fujii, Makoto| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 2004-12-01
Publication Title 岡大三朝医療センター研究報告
Volume volume75
Content Type Departmental Bulletin Paper
Author Okamoto, Makoto| Ashida, Kozo| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Nishida, Norikazu| Nagata, Takuya| Yokoi, Tadashi| Takata, Shingo| Tanizaki, Yoshiro| Tnimoto, Mitsune|
Published Date 2003-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/14909
Title Alternative 脂肪の変化と関連した気管支喘息に対するn-3系脂肪酸食の効果 ― n-6系脂肪酸との比較
FullText URL 071_050_058.pdf
Author Okamoto, Makoto| Mitsunobu, Fumihiro| Ashida, Kozo| Mifune, Takashi| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Takata, Shingo| Tanizaki, Yoshiro| Harada, Mine|
Abstract N-3 fatty acids have been reported to be effective for asthma. In the present study, the effects of perilla seed oil (n-3 fatty acids) on asthma were compared with the effects of corn oil (n-6 fatty acids) in terms of pulmonary function, lipometabolism and the generation of leukotriene C4 (LTC4) by leucocytes. A total of 28 asthmatic patients were randomly divided into two groups : Group A patients (15 subjects) consumed perilla seed oil-rich supplementation, while Group B patients (13 subjects) consumed corn oil-rich supplementation for 4 weeks. Generation of LTC4 by leucocytes, respiratory function and the serum levels of lipids were compared between the two groups. The generation of LTC4 by leucocytes decreased significantly in Group A subjects following perilla seed oil-rich supplementation for 2(P < 0.05) and 4 weeks(P < 0.01). A significant difference in the generation of LTC4 was observed between the two groups after different dietary supplementations for 4 weeks (P < 0.05). Significantly increased values for PEF (P < 0.01), FVC (P < 0.05) and FEV1.0 (P < 0.05) were found in Group A subjects following perilla seed oil supplementation for 4 weeks, compared with the initial value prior to supplementation. A significant decrease in the serum level of total cholesterol, LDL-cholesterol and phospholipid was detected in Group A subjects following perilla seed oil supplementation for 4 weeks. The present results suggest that peril la seed oil-rich supplementation is effective in the treatment of asthma in terms of its ability to suppress LTC4 generat ion by leucocytes, and in inducing an improvement in pulmonary function associated with changes in the serum level of lipids.
Abstract Alternative n-3系脂肪酸は喘息に有効であることが報告されている。今回、エゴマ油(n-3系脂肪酸)の喘息に対する効果を呼吸機能、イコトリエンC4(LTC4)産生能の観点から、コーン油(n-6系脂肪酸)のそれと比較した。28例の喘息患者を無作為に2群に分け、A群(15例) はエゴマ油食を、B群(13例)はコーン油食をそれぞれ4週間摂取した。白血球LTC4産生能、呼吸機能、血清脂質を2群間で比較した。白血球LTC4産生能はエゴマ抽食を摂取したA群で2週後(P < 0.05)、4過後(P < 0.01)に有意に低下した。食事摂取4過後に岳群間でLTC4産生能に有意差が認められた(P < 0.05)。エゴマ油食を4過摂取したA群ではピークフロー(P < 0.05)、肺活量(P < 0.01)、1秒量(P < 0.05)が食事摂取前に比し、有意に増加した。またA群ではエゴマ坤食摂取4週後に血清総コレステロール値、LDL-コレステロール値、リン脂質の有意な低下がみられた。こ れらの結果より、エゴマ油食は白血球LTC4産生能を抑制することにより、呼吸機能を改善し、血清脂質の変化と関連して、喘息の治療に有効であることが示唆された。
Keywords perilla seed oil (エゴマ抽) a -1inolenic acid (α-リノレン酸) leukotriene C4 (ロイコトリエンC4) respiratory function (呼吸機能) 脂質代謝
Publication Title 岡大三朝分院研究報告
Published Date 2001-02-01
Volume volume71
Start Page 50
End Page 58
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308088
Author Ashida, Kozo| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Nishida, Norikazu| Nagata, Takuya| Takata, Shingo| Yokoi, Tadashi| Tanizaki, Yoshiro|
Published Date 2003-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/14839
Title Alternative 脂質代謝に関連した気管支喘息患者における白血球ロイコトリエン産生能に対するα-リノレン酸食の効果
FullText URL 72_045_054.pdf
Author Okamoto, Makoto| Mitsunobu, Fumihiro| Ashida, Kozo| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Nishida, Norikazu| Yokoi, Tadashi| Takata, Shingo| Tanizaki, Yoshiro| Tanimoto, Mitsune|
Abstract Dietary sources of a -linolenic acid, such as perilla seed oil, may have the capacity to inhibit the generation of leukotrienes (LTs) by leucocytes in patients with asthma, as has been reported with the consumption of other long - chain n- 3 fatty a-cids. The factors affecting the suppression of leukotriene (LT) C4 generation by leucocytes were examined by comparing the clinical features of patients with asthma who had been given dietary perilla seed oil (n - 3 fatty acids). Group A consisted of patients in whom the leucocyte generation of dietary perilla seed oil LTC4 was suppressed by this procedure. Group B consisted of those in whom LTC4 generation was not suppressed. LTC4 generation by leucocytes significantly decreased in group A for two (P<0.05) and four weeks (P<O. OS), conversely, significantly increased in group B for four weeks (p<O. OS). The two study groups differed significantly in LTC4 generation by leucocytes after four weeks of dietary supplementation (P<0.05). Ventilatory parameters such as peak expiratory flow (PEF) , forced vital capacity (FVC) and forced expiratory volume in one second (FEV(1.O)) increased significantly after four weeks of dietary supplementation in group A (P<0.05). Values of PEF, FVC, FEV(1.O) and V(25) between groups A and B significantly differed prior to dietary supplementation. Serum levels of total cholesterol, LDL- cholesterol and phospholipid were significantly decreased by dietary supplementation in group A after four weeks. Serum levels of total-choles terol, triglyceride, HDL-Cholesterol, LDL-Cholesterol and phospholipid values between the two study groups differed significantly prior to dietary supplementation. Serum levels of triglyceride and LDL- cholesterol differed significantly between the two study groups after four weeks of dietary supplementation. The effects of dietary supplementation with perilla seed oil to patients with asthma by suppressing the generation of LTC4 is associated with clinical features such as respiratory function and lipometabolism.
Abstract Alternative エゴマ油のようなα-リノレン酸食が他のn-3系不飽和脂肪酸食において報告されてきた様に喘息患者の白血球ロイコトリエン(LTs)産生能を抑制すると考えられる。そこでエゴマ油(n-3系脂肪酸)を摂取した気管支喘息患者の臨床所見を比較することによって白血球ロイコトリエン(LT)C4の抑制に影響する因子を検討した。A群はエゴマ油摂取により白血球LTC4の産生能が抑制された群であり、B群は白血球LTC4の産生能が抑制されなかった群である。A群では食事摂取2週後(P<0.05),4週後(P<0.05)に白血球LTC4産生能が低下した。逆にB群では摂取4週後有意に増加した(P<0.05)0 2群間で食事摂取4遡後に白血球LTC4産生能に有意差がみられた(P<0.05)。ピークフロー値(PEF)、努力性肺活量(FVC)、1秒量(FEV(1))といった呼吸機能はA群において食事摂取4週後に有意に上昇した(P<0.05)。食事摂取前のPEF、FVC、FEV(1)、V(25)はA群,B群の2群間で有意差がみられた。A群において血清総コレステロール、低比重リポ蛋白(LDL)コレステロール、リン脂質は食事摂取4週後に有意に低下した。食事摂取前の血清総コレステロール、中性脂肪、高比重リポ蛋白コレステロール、LDLコレステロール、リン脂質において2群間に有意差がみられた。血清中性脂肪、LDLコレステロールは食事摂取4週後2群間に有意差がみられた。気管支喘息患者のある群へのエゴマ油食はLTC4産生能を抑制し、それには呼吸機能や脂質代謝といった臨床因子が関連していると考えられた。
Keywords alpha -linolenic acid leukotrieneC4 bronchial asthma lipometabolism
Publication Title 岡大三朝分院研究報告
Published Date 2002-02-01
Volume volume72
Start Page 45
End Page 54
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308212
Author Mitsunobu, Fumihiro| Ashida, Kozo| Hosaki, Yasuhiro| Tsugeno, Hirofumi| Nishida, Norikazu| Nagata, Takuya| Yokoi, Tadashi| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 2003-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/14835
Title Alternative 気管支喘息における気管支肺胞洗浄液中の細胞成分に対する加齢および副腎皮質ホルモンの影響
FullText URL 72_016_022.pdf
Author Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Nishida, Norikazu| Takata, Shingo| Yokoi, Tadashi| Tanizaki, Yoshiro|
Abstract Effects of aging and glucocorticoid therapy on bronchoalveolar lavage (BAL) cells, particularly lymphocytes, neutrophils and eosinophils, were examined in 81 patients with asthma. 1. The proportion of BAL lymphocytes tended to increase with aging in asthmatics under age 69 years, and the proportion was significantly higher in patients with asthma between the ages of 60 and 69 than in those under age 39 and between the ages of 40 and 49. 2. The proportions of BAL neutrophils and eosinophils were not related to aging. 3. The proportion of BAL lymphocytes was higher in patients without glucocorticoid therapy than in those with steroid-dependent intractable asthma (SOIA) in those under age 69 years. In patients between the ages of 50 and 59, the proportion of BAL lymphocytes was significantly higher in patients without glucocorticoids than in those with SOIA. 4. The proportion of BAL neutrophils was higher in patients without glucocorticoid therapy than in those with SOIA, and the difference was significant in patients between the ages of 60 and 69. 5. The proportion of BAL eosinophils was not related to glucocorticoid therapy. These results suggest that the proportion of BAL lymphocytes and neutrophils is affected by aging and glucocorticoid therapy, but not BAL eosinophils.
Abstract Alternative 気管支喘息81例を対象に,気管支肺胞洗浄(BAL)液中の細胞成分,特にリンパ球,好中球および好酸球に対する加齢および副腎皮質ホルモ ンの影響について検討を加えた。 1.BAL液中のリンパ球頻度は,69才以下の症例では加齢とともに増加する傾向を示し,60-69才の年齢層では,39才以下および40-49才の年齢層の症例に比べ有意に高い値を示した。  2.BAL液中の好中球および好酸球頻度と加齢との間には関連が見られなかった。  3.BAL液中のリンパ球頻度は,69才以下の症例では,ステロイド依存性重症難治性喘息(SDIA)に比べ,ステロイド非使用例で有意に高い値を示し,50-59才の年齢層ではその差は有意であった。  4.BAL液中好中球頻度は,ステロイド非使用例に比べSDIA症例において高い値を示し,60-69才の年齢層ではその差は有意であった。  5.BAL液中好酸球頻度は,ステロイド使用の有無との関連は見られなかった。これらの結果は,BAL液中リンパ球および好中球頻度は加齢や副腎皮質ホルモン投与の影響を受けるが,BAL液中好酸球額度には影響しないことを示唆している。
Keywords aging asthma glucocorticoid therapy BAL cells
Publication Title 岡大三朝分院研究報告
Published Date 2002-02-01
Volume volume72
Start Page 16
End Page 22
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308605
Author Kikuchi, Hiroshi| Shiozawa, Nobuyoshi| Takata, Shingo| Ashida, Kozo| Mitsunobu, Fumihiro|
Published Date 2013-12-12
Publication Title International Journal of Chronic Obstructive Pulmonary Disease
Volume volume9
Content Type Journal Article
JaLCDOI 10.18926/14908
Title Alternative 寒冷負荷の末梢循環に及ぼす影響
FullText URL 071_042_049.pdf
Author Hosaki, Yasuhiro| Mitsunobu, Fumihiro| Mifune, Takashi| Ashida, Kozo| Tsugeno, Hirofumi| Okamoto, Makoto| Takata, Shingo| Yokoi, Tadashi| Tanizaki, Yoshiro| Ochi, Koji| Tsuji, Takao|
Abstract The purpose of this study is the effect of partial cold-loading on whole body peripheral circulation. The body surface peripheral circulation in 24 cases (25 years old to 86 years old, the average was 64.3 years) who were suffering coldness, numbness or pain in their feet was examined using Laser-Doppler blood flowmetry. The peripheral circulation at the base of the 2nd toe of the right foot was estimated after the foot was submerged and cooled for 5 min in a water bath at 20°C (i. e. cold-loading). At the same time, the distant body surface peripheral circulation was estimated at the base of the 2nd finger of the right hand. Simultaneous observations were made of blood flow, blood mass and blood velocity. The peripheral blood flow of the upper limbs at a room temperature of 20°C (pre-loading) was 5.00 ml/min 100 9 tissue on average. The average blood rnass was 287 and the average blood velocity was 0.516. On the other hand, the average peripheral blood flow of the lower limbs was 2.23, the average blood mass was 149 and the average blood velocity was 0.574. This result shows that the blood flow and blood mass of the upper limbs were more than in the lower limbs. The average blood flow at the upper limbs decreased to 3.69 from 5.00 (or at 26.2% ) when the lower limbs were submerged and cooled for 5 min in a water bath at 20°C (i. e. cold-loading). On the other hand, blood flow of the lower limb was 1.51 m l under a condition of cold-loading, and decreased 32.3% from 2.23. Blood mass of the upper limb was 241 on average and decreased 16.0% from 287. Blood mass of the lower limb was 113 on average and decreased 24.2% from 149. There was no difference between blood velocity of the upper limb under the conditions of room temperature at 20°C (0.516) and cold-loading (0.501). However, blood velocity of the lower limb increased to 0.642 from 0.574. After the end of cold loading, there was some tendency for blood flow, blood rnass and blood velocity to return to the amount at pre-loading. Twenty minutes after the end of cold-loading, blood flow increased to 5.74 (14.8% ) compared with pre-loading (5.00). However, blood flow of the lower limb remained at only 83.9% (1.87) of the amount at pre-loading (2.23). These results show that partial cold-loading of the lower limb had a quantitative effect on the distant peripheral circulation. The speculated mechanism of this phenomenon is that it to protects against loosing body heat from the body surface under the conditions of local cold-loading. Our body has defense mechanisms to decrease whole body peripheral circulation to protect against loosing body heat.
Abstract Alternative 局所寒冷負荷の全身の末梢循環に及ぼす影響について検討する目的で,下肢の20℃冷水負荷時における上肢末梢循環の変化をレーザードプラー血流計を用いて数量的に測定し検討を行なった。症例は,下肢に冷え症,しびれ感を有した25歳から86歳までの24症例(平均年齢64.3歳)であった。下肢,上肢の末梢循環を20℃冷水負荷時に,末梢血流量,血液量,血流速度について観察した。上肢の末梢血流量は20℃室内安静時平均5.00mβ/min/100g tissue,血液量は287,血流速度は0.516であった。下肢は血流量2.23,血液量は149,血流速度は0.574であった。上肢の血流量,血液量ともに下肢より多いことが示された。しかし同一症例における上肢と下肢との間の血流量,血液量,血流速度に相関関係は認めなかった。下肢の20℃ 冷水負荷中の上肢の血流量は平均3.69で有意に低下(26.2%)した。下肢はその間1.51で有意に低下(32.3%)した。上肢の血液量は平均241で有意に低下(16.0%)した。下肢はその間113で有 意に低下(24.2%)した。上肢の血流速度は平均0.501で不変であった。下肢はその間0.642で有意に増加(11.8%)した。冷水負荷直後よりそれぞれ負荷前に復帰する傾向を示した。負荷後20分では上肢の血流量は平均5.74で負荷前値に比べて増加(14.8%)傾向を示した。下肢は1.87で負荷前値に比べて低値(83.9%)に留まる傾向にあった。下肢の冷水負荷は上肢の末梢循環にも影響を及ぼすことが数量的に示された。局所の寒冷負荷による体温の低下を防ぐために反射的に全身の皮膚の末梢循環量を低下させていることが示された。
Keywords Laser-Doppler blood flowmetry (レーザードプラー血流計) cold loading (冷水負荷) peripheral circulation (末梢循環) blood flow (血流量) blood velocity (血流速度)
Publication Title 岡大三朝分院研究報告
Published Date 2001-02-01
Volume volume71
Start Page 42
End Page 49
ISSN 0918-7839
language 日本語
File Version publisher
NAID 120002308072
JaLCDOI 10.18926/15001
Title Alternative アトピー型および非アトピー型気管支喘息の発作発症機序の差異について・・・ロイコトリエンC4の役割について
FullText URL 067_021_027.pdf
Author Ashida, Kozo| Mifune, Takashi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Yokota, Satoshi| Tsugeno, Hirofumi| Tanizaki, Yoshiro|
Abstract Concentrations of main bronchoconstricting chemical mediators, histamine and leukotriene C4 (LTC4), were measured in bronchoalveolar lavage (BAL) fluid, and when cells (peripheral leukocytes and BAL cells) were stimulated by Ca ionophore A23187, in 7 atopic and 7 nonatopic asthma patients. 1. The proportion of basophilic cells was significantly larger in atopic than in nonatopic asthma (p<0.05), however no significant difference was present in the other BAL cells between the two asthma types. 2. Concentration of histamine in BAL fluid was significantly higher in atopic than that in nonatopic asthma, however, difference in that of LTC4 was not found between them. 4. The release of LTC 4 from BAL cells was higher in nonatopic than that in atopic asthma, but this was not significant. In contrast, the release of histamine was significantly higher in atopic compared to that in nonatopic asthma (p<0.001) when the cells were stimulated by Ca ionophore A23187. These results suggest that both histamine and LTC4 participate in the onset mechanism of atopic asthma, and only LTC4 in that of nonatopic asthma.
Abstract Alternative 気管支喘息患者14人(アトピー型,非アトピー型各7人)につき気管支肺胞洗浄(BAL)液及び気管支肺胞洗浄細胞と末梢血白血球をカルシウムイオノファA23187で刺激し,主要な気管支収縮メディエーターであるヒスタミンとロイコトリエンC4(LTC4)の濃度を測定した。1.BAL中細胞の比率では好塩基球のみ非アトピー型に比べアトピー型で優位に高い値であった。(p<0.05) 2.BAL液のヒスタミンの濃度はアトピー型で有意に高い値であったが,ロイコトリエンC4はアトピー型,非アトピー型で有意な差を認めなかった。3.BAL細胞からのカルシウムイオノファA23187刺激によるロイコトリエンC4産生はアトピー型に比べ非アトピー型で高い値であったが,有意差は認めなかった。一方,同刺激によるヒスタミン遊離は非アトピー型よりアトピー型で有意 に高値であった。(p<0.001)。以上の結果よりアトピ-型の気管支喘息の発症機序にはヒスタミン,ロイコトリエンC4の両者が,非アトピー型に於いてはロイコトリエンC4のみが主として関与していること可能性が示唆された。
Keywords Histamine LTC4 atopic nonatopic BAL cells
Publication Title 岡大三朝分院研究報告
Published Date 1996-11
Volume volume67
Start Page 21
End Page 27
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307446
JaLCDOI 10.18926/15000
Title Alternative Ⅱ型(細気管支閉塞)喘息と閉塞性細気管支炎における細気管支領域の炎症反応の差
FullText URL 067_014_020.pdf
Author Mifune, Takashi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Yokota, Satoshi| Tsugeno, Hirofumi| Takeuchi, Kazuaki| Nawa, Yuichiro| Tanizaki, Yoshiro| Tada, Shinya| Harada, Mine|
Abstract Ventilatory function and inflammatory cells in airways were compared between patients with type Ⅱ (bronchiolar obstruction) asthma and those with obstructive bronchiolitis. 1. Age and age at onset were higher in patients with type Ⅱ asthma than in those with obstructive bronchiolitis. IgE-mediated allergic reaction was observed in patients with type Ⅱ asthma, but not in those with obstructive bronchiolitis. 2. In ventilatory function tests, all ventilatory parameters examined were lower in patients with type Ⅱ asthma compared to those with obstructive bronchiolitis, and the differences were significant in FEV1.0% (p<0.001), % MMF (p<0.02), and V50 (p<0.01). 3. The proportion of BAL neutrophils was very high in type Ⅱ asthma (55.7%) and obstructive bronchiolitis (74.4%), however, this was not significant. 4. Absolute numbers/BAL fluid of total cells, BAL macrophages and BAL neutrophils were significantly higher in patients with obstructive bronchiolitis than in those with type Ⅱ asthma. 5. The results on absolute number/mℓ of BAL cells demonstrated that number of BAL neutrophils markedly larger in patients with obstructive bronchiolitis compared to those with type Ⅱ asthma. These results show that high proportion of BAL neutrophils was observed in the two respiratory diseases, however, the degree of inflammation in airways was markedly greater in obstructive bronchiolitis.
Abstract Alternative Ⅱ型喘息と閉塞性細気管支炎の臨床的特徴について,換気機能および気道炎症反応を中心に検討を加えた。1.年齢,および発症年齢とも閉塞性細気管支炎に比べⅡ型喘息において高い傾向が見られた。IgE系反応はⅡ型喘息では観察されたが,閉塞性細気管支炎では見られなかった。2.換気機能では,測定された全ての換気パラメーターにおいて,その値は閉塞性細気管支炎に比べⅡ型喘息でより低い値を示し,FEV1.0%,% MMFおよび% V50では有意の差が見られた。3.BAL液中好中球頻度は,Ⅱ型喘息(55.7%),閉塞性細気管支炎(74.4%)いずれにおいても高い値を示したが,両者間に有意の差は見られなかった。4.一方,BAL液中の絶対数では,Ⅱ型喘息に比べ,閉塞性細気管支炎において,総細胞数,マクロファージおよび好中球数が有意の高値を示した。5.また,lmℓあたりの細胞数の比較でも,閉塞性細気管支炎で好中球数が著明な高値を示した。これらの結果より,この2疾患では気道内好中球増多は同様に見られるものの,その気道炎症の程度は明らかに閉塞性細気管支炎でより高度であることが示された。
Keywords Ⅱ型喘息 (Type Ⅱasthma) 閉塞性細気管支炎 (Obstructive bronchiolitis) 換気機能 (Ventilatory function) BAL好中球 (BAL neutrophiIs) IgE系反応 (IgE-mediated allergy)
Publication Title 岡大三朝分院研究報告
Published Date 1996-11
Volume volume67
Start Page 14
End Page 20
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307471
Author Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Iwagaki, Naofumi| Nagata, Takuya| Fujii, Makoto| Takata, Shingo| Yokoi, Tadashi| Hamada, Masanori| Tanizaki, Yoshiro|
Published Date 2004-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume74
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/15006
Title Alternative 気管支喘息におけるエゴマ油と白血球のロイコトリエンB4,C4合成
FullText URL 067_035_042.pdf
Author Ashida, Kozo| Mifune, Takashi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Yokota, Satoshi| Tsugeno, Hirofumi| Tanizaki, Yoshiro| Yamamoto, Junko| Tsuji, Takao|
Abstract In recent years, it has been noted that there is a close correlation between leukotrienes and late asthmatic reaction (LAR). In this study, effects of dietary supplementation with perilla seed oil rich in alpha-linolenic acid, which is speculated to affect the generation of leukotrienes through metabolism of arachidonic acid (AA), were evaluated in 6 patients with asthma. The symptoms and ventilatory function were improved after 2-week dietary supplementation with perilla seed oil. The generation of LTB4 and LTC4 by peripheral leucocytes stimulated with Ca ionophore A23187 was significantly suppressed by the dietary supplementation (LTB4 and LTC4 ; p<0.05). Regarding the composition of fatty acids in serum phospholipids, the concentrations of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and AA tended to increase after the supplementation, accompanied with an increase in the ratio of EPA to AA. These results suggest that dietary supplementation with perilla seed oil brings beneficial effects in the treatment of asthma.
Abstract Alternative 近年,ロイコトリエンと遅発型気管支反応(LAR)との密接な関連が注目されている。本論文では,アラキドン酸(AA)代謝を通してロイコトリエン合成に関与すると推定される,αリノレン酸を多く含むエゴマ油による食事療法の臨床効果を,気管支喘息を対象に検討した。臨床症状および換気機能は,2週間のエゴマ油投与で明らかな改善差傾向を示した。Ca ionophore A23187刺激時の白血球のLTB4およびLTC4産生は,エゴマ油投与により,投与前に比べ有意の減少を示した(p<0.05)。血中脂肪酸に関しては,イエイコサペンタエン酸(EPA),ドコサヘキサエン酸(DHA)およびAA濃度は,エゴマ油投与により増加傾向を示し,同時にEPA/AA比も増加する傾向が見られた。以上の結果より,エゴマ油による食事療法は,治療上有用であると考えられた。
Keywords n-3系脂肪酸 (n-3 fatty acids) 気管支喘息 (bronchial asthma) アラキドン酸 (arachidonic acid) LTB4 LTC4
Publication Title 岡大三朝分院研究報告
Published Date 1996-11
Volume volume67
Start Page 35
End Page 42
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002307804
JaLCDOI 10.18926/14986
Title Alternative 気管支喘息患者における腰椎海綿骨骨塩量と脊椎圧迫骨折の臨床的特徴
FullText URL 068_045_050.pdf
Author Tsugeno, Hirofumi| Nakai, Mutsuro| Okamoto, Makoto| Mifune, Takashi| Mitsunobu, Fumihiro| Ashida, Kozo| Hosaki, Yasuhiro| Akiyama, Tsuneo| Tsuji, Takao| Tanizaki, Yoshiro|
Abstract Clinical risk factors associated with the development of osteoporosis and vertebral fractures were evaluated in patients with asthma in relation to sex, age, and dose of glucocorticoids (GC). In 75 asthmatic patients including 44 steroid-dependent asthma, the bone mineral density (BMD) of the lumber spines was measured by quantitative computed tomography (QCT). Thirty five patients of them were followed up with radiographs over a period of 0.5 to 4 years (average: 2.6±1.3 years). The BMD was significantly lower in older (p<O.01) or female (p<0.05) patients. All the five patients developing vertebral compression fractures were female and more than 64 y.o., and received systemic glucocorticoid (GC) therapy for more than 3 years with a lot of cumulative gramdosage of GC. No significant correlation was demonstrated between the BMD and the dose of systemic GC per day, but multiple regression analysis demonstrated a significant relationship (p<O.o1) between the BMD and lifetime cumulative gramdosage of GC. Multiple regression analysis also demonstrated significant relationships (p<O.01) between the BMD and clinical factors such as age and sex. These results indicates that the bone loss and vertebral fractures of patients with asthma are influenced by the patient's age, sex, and the lifetime cumulative GC dose.
Abstract Alternative 対象は気管支喘息75症例。このうち44例はステロイド依存性難治症例であった。35症例については,0.5年から4年間(平均:2.6±1.3年間)の経時的観察もおこなわれた。これらの症例の骨塩量に影響を及ぼす因子について検討をおこなった。高齢者,女性に有意な低骨塩量を認めた。重回帰分析にて年齢,性別,経口副腎ステロイド投与総量などの項目に骨塩量と有意な関連が認められた。また,35症例中5例に脊椎圧迫骨折が発生し,いずれも骨塩量が低く,高齢者,女性,長期ステロイド内服例であった。これらのことから気管支喘息患者においては,女性,高齢者,長期ステロイド内服例に骨塩量減少や脊椎圧迫骨折のリスクが高いと考えられた。また,ステロイド続発性骨粗鬆症の発生には,ステロイドの現在の一日内服量よりもこれまでの総積算内服量が重要と考えられた。
Keywords 気管支喘息 (bronchial asthma) 骨粗鬆症 (osteoporosis) 椎体骨折 (vertebral fracture) ステロイド療法 (glucocorticoid therapy)
Publication Title 岡大三朝分院研究報告
Published Date 1997-12
Volume volume68
Start Page 45
End Page 50
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308467
Author Tanizaki, Yoshiro| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Ashida, Kozo| Hamada, Masanori| Iwagaki, Naofumi| Fujii, Makoto| Takata, Shingo|
Published Date 2004-12-01
Publication Title 岡大三朝医療センター研究報告
Volume volume75
Content Type Departmental Bulletin Paper
JaLCDOI 10.18926/14934
Title Alternative 気管支喘息症例における皮質骨海綿化
FullText URL 070_053_060.pdf
Author Tsugeno, Hirofumi| Nakai, Mutsuro| Okamoto, Makoto| Harada, Seishi| Takata, Shingo| Mifune, Takashi| Mitsunobu, Fumihiro| Ashida, Kozo| Hosaki, Yasuhiro| Akiyama, Tsuneo| Tsuji, Takao| Tanizaki, Yoshiro|
Abstract In previous studies, we have demonstrated that chronic administration of systemic glucocorticoids decreases cortical bone mineral density (BMD), cortical bone volume, bone strength, and induces development of pathologic fractures in asthmatic patients. We have also demonstrated that glucocorticoid administration appears to be responsible for the process of cortical bone porosity at both endosteal and intracortical sites in postmenopausal asthmatic patients. There is a difference of gonadal hormones between male and female. To investigate the influence of hormonal difference on glucocorticoid-induced cortical bone porosity, we studied cortical bone volume and BMD in both male and female patients with asthma in this report. A total of 99 asthmatic patients (male 26 cases, female 73 cases) were enrolled in the study. Peripheral quantitative computed tomography (pQCT) was used to measure cortical BMD and relative cortical volume. The cortical volume-density relationship appeared to remain constant regardless of the level of systemic glucocorticoid administration, age or sex, suggesting cortical bone porosity causes similar and simultaneous decreases in cortical bone volume and density. In conclusion, glucocorticoid administration appears to be responsible for the process of cortical bone porosity at both endosteal and intracortical sites despite the gonadal hormonal differences.
Abstract Alternative 【目的】これまでに我々は,気管支喘息症例において経口ステロイドによる皮質骨骨密度,容積の減少が骨折に関与する新知見を報告し,閉経後女性では皮質骨骨密度一容積の減少はステロイド投与量にかかわらず一定であることを報告してきた。この皮質骨骨密度-容積の関係において性差による違いを検討するために,男性,女性患者の両方について検討を行った。【方法】対象はステロイド依存性喘息99例(男性26例,女性73例)。性別,年齢,経口ステロイド積算総投与量により6群に分類した。椎体圧迫骨折はⅩ線側面像にて評価 し,皮質骨容積比および皮質骨骨密度はpQCT(Stratec XCT960)を用いて測定した。それぞれの群の皮質骨骨密度-容積比の関係を算出し比較検定をおこなった。【結果】それぞれの群の皮質骨の骨密度と容積比は有意に相関した。それぞれの群の皮質骨骨密度-容積比の傾きは,いずれも有意差を認めなかった。【結論】気管支喘息症例におけるステロイド投与による皮質骨の骨密度と容積の減少は,性別にかかわらずほぼ一定で,皮質骨は内側と外側において同様に海綿化してゆくと考えられた。
Keywords bronchial asthma systemic glucocorticoid cortical bone cortical porosity peripheral quantitative computed tomography
Publication Title 岡大三朝分院研究報告
Published Date 1999-12
Volume volume70
Start Page 53
End Page 60
ISSN 0918-7839
language 英語
File Version publisher
NAID 120002308477