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ID 19060
Eprint ID
19060
Title Alternative
Studies on the Virus of Infectious Hepatitis in Okayama Prefecture Ⅰ: Purification of the Isolated Virus Ⅱ: Electron Microscopic Study on the Isolated Virus
Author
Hashimoto, Ryohei
Abstract Alternative
Purification and electron microscopic study of the virus isolated by Murakami et al. from the patients of infectious hepatitis in Okayama Prefecture were tried in parallel with animal experiments. The results are summarized as follows: 1) Of all the purification methods tried, the combination of infusion with a saline solution and centrifugation was proved to be the most excellent one for electron microscopic observation and animal experiments. The good efficiency and the simplicity of its procedures also made this method suitable for the purification of the virus of infectious hepatitis. 2) In electron micrographs, the viruses were observed as round or ovoid particles of abo t 50 mμ in diameter. 3) Inoculation of the material containing these particles into mice caused such pathological findings as previously reported by Murakami at el.; a high degree of degeneration or necrosis of liver parenchymal cells accompanied with various cell infiltrations. These observations led the author to the conclusion that the purified particles were nothing but the isolated hepatotropic viruses.
Published Date
1958
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume70
Issue
issue7
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
2307
End Page
2320
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/70/7/70_7_2307/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
File Version
publisher
Refereed
True
Eprints Journal Name
joma