岡山医学会 Acta Medica Okayama 0030-1558 94 3-4 1982 二価鉄誘導脂質過酸化反応に関する研究 第一編 二価鉄誘導脂質過酸化反応溶液中の二価鉄の定量 349 357 EN Keizi Hashimoto The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe(�U). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe(�U) quantitatively and selectively the system of Fe(�U)-induced lipid peroxidation, and obtained the following results. 1) Fe(�U) could be measured quantitatively without reductant. The Fe(�U) complex had a molar absorptivity of 4.5×10(4)dm(3) mol(-1) cm(-1) at 756 nm. 2) Fe(�U) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe(�U) complex had a molar absorptivity of 3.14×10(4)dm(3) mol(-1) at 756nm. 3) Fe(�U) could be measured quantitatively and selectivily under conditions involving a mixture of Fe(�U). No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 94 3-4 1982 二価鉄誘導脂質過酸化反応に関する研究 第二編 二価鉄誘導脂質過酸化反応における誘導期(lag)の二価鉄の消長による解析 359 368 EN Keizi Hashimoto The author measured simultaneously Fe(�U)-induced peroxidation of lipid extracted from rat liver mitochondria by the TBA method and the concentration of Fe(�U) added to the medium quantitatively using Nitoroso-PSAP reagent. 1) An induction period (lag) at the early phase of the reaction of Fe(�U)-induced lipid peroxidation was observed as well as in mitochondria. 2) After most of the Fe(�U) concentration had decreased, the TBA-value, after lagtime, increased sharply. 3) Phosphate added to the medium shortened the lag-time. The lag-time in the presence of phosphate varied with the concentration and pH. However, in each case, there was a correlation between the decrement of Fe(�U) concentration and the length of lag time as described above. 4) By adding EDTA for various reaction times, Fe was chelated immediately, then the TBA value did not increase. 5) The results suggest: iron interacted with the chain reaction of lipid peroxidation, mainly as Fe(�U) and/or partially Fe(�V); Fe(�U) maintains the lag as a inhibitory action to the resolution into malondialdehyde(MDA) from lipid peroxide; Fe(�V) was required for the formation of MDA. 6) Using these results, the author discussed the mechanisms of Fe(�U)-induced lipid peroxidation. No potential conflict of interest relevant to this article was reported.