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ID 16138
Eprint ID
16138
FullText URL
Thumnail 97_201.pdf 450 KB
Title Alternative
Ferrous ion-induced lipid peroxidation Part I. Analysis of the lag period in liver mitochondria of whole-body irradiated rats.
Author
Morimoto, Setsuo
Abstract
Fe(II)-induced lipid peroxidation was measured at 25℃ by the TBA method in rat liver mitochondria 3 days after 800R of whole-body irradiation, and decrements in Fe(II) concentrations in the medium were measured by the Nitroso-PSAP method, simultaneously. An induction period (lag) was observed in the reaction curve of the Fe(II)-induced lipid peroxidation. The Fe(II) concentration decreased gradually during the lag period, but decreased rapidly thereafter. The oxidating velocity of Fe(II) in the presence of mitochondria was significant compared with the Fe(II) auto-oxidation in the mixture. At a given dose of Fe (II), a greater amount of mitochondrial protein allowed a shortened lag and accelerated the oxidating velocity of Fe (II). The relationship between MDA formation per unit protein and lag time showed the same tendency, so that MDA formation increased with a lengthened lag. With given doses of protein (0.5mg/ml) and Fe(II), a shorter lag and greater MDA formation were found in irradiated rat liver mitochondria than in non-irradiated rat liver mitochondria. With the addition of Fe(II) (0.10mM), the lag was lengthened and MDA formation increased the same in irradiated as in non-irradiated mitochondria, compared with Fe(II) (0.05mM).
Keywords
lipid peroxidation
ferrous ion
irradiation
mitochondria
Published Date
1985-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume97
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
201
End Page
209
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/97/3-4/97_3-4_201/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma