Author Matsubara, Fumie| Endo, Hiroi| Sudo, Michiyasu| Tanizaki, Yoshiro|
Published Date 1989-07
Publication Title 環境病態研報告
Volume volume60
Content Type Data or Dataset
Author Matsubara, Fumie| Sudo, Michiyasu| Tanizaki, Yoshiro|
Published Date 1988-08
Publication Title 環境病態研報告
Volume volume59
Content Type Data or Dataset
JaLCDOI 10.18926/19758
Title Alternative Separation of paripheral leukocytes by counterflow centrifugation elutriation
FullText URL 059_037_041.pdf
Author Sudo, Michiyasu| Matsubara, Fumie| Araki, Hiroyuki| Kitani, Hikaru| Tanizaki, Yoshiro|
Abstract JE-6B rotor(Beckman)を用いてcounterflow centrifugation elutriationによる末梢血白血球の分離を試み,若干の検討を加えた。1.flow rateが低い場合はリンパ球が大部分を占めたが,flow rateの増加とともに頻度は減少した。2. flow rateの増加とともに,好塩基球の頻度が増加し,9-10ml/minでは頻度のみでなく細胞数の増加も認められた。各fractionにおけるヒスタミン含有量も同様の傾向が認められた。3.好中球のpeakはflow rate 13ml/minであり以後減少を示した。4.最終段階で好酸球が増加傾向を示しChamber内で最も高値を示した。以上の結果よりcounterflow centrifugation elutriationによる末梢血白血球細胞分離は,in vitroにおける実験に有用な手段である可能性が示唆された。
Abstract Alternative Separation of peripheral leukocytes was carried out in 5 healthy subjects by counterflow centrifugation elutriation with JE-6B rotor. 1. Number of lymphocytes was predominant at low flow rate (4.5ml/min) of counterflow centrifugation elutriation. 2. As flow rate was increased (9-10ml/min), the purity and number of basophils, and histamine content in the fraction were gradually increased. 3. The purity and number of neutrophils were gradually increased, and the purity was reached the peak at 13ml/min. 4. The purity and number of eosinophils were gradually increased at the late stage of the centrifugation elutriation. The results obtained here might suggest that cell separation by counterflow centrifugation elutriation is more useful for experiments, especially allergy testing, in vitro.
Keywords 逆流負荷遠心法 (Counterflow centrifugation elutriation) JE-6Bローター (JE-6B rotor) 細胞分離 (Cell separation) 好塩基球 (Basophils)
Publication Title 環境病態研報告
Published Date 1988-08
Volume volume59
Start Page 37
End Page 41
ISSN 09133771
language 日本語
File Version publisher
NAID 120002309189
Author Matsubara, Fumie| Sudo, Michiyasu| Tanizaki, Yoshiro| Morinaga, Hiroshi|
Published Date 1987-08
Publication Title 環境病態研報告
Volume volume58
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| Kitani, Hikaru| Tanizaki, Yoshiro|
Published Date 1994-09
Publication Title 岡大三朝分院研究報告
Volume volume65
Content Type Data or Dataset
Author Matsubara, Fumie| Endou, Hiroi| Kitani, Hikaru| Tanizaki, Yoshiro|
Published Date 1992-06
Publication Title 岡大三朝分院研究報告
Volume volume63
Content Type Data or Dataset
Author Matsubara, Fumie| Endou, Hiroi| Inoue, Taeko| Kitani, Hikaru| Tanizaki, Yoshiro|
Published Date 1993-06
Publication Title 岡大三朝分院研究報告
Volume volume64
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1995-09
Publication Title 岡大三朝分院研究報告
Volume volume66
Content Type Data or Dataset
Author Matsubara, Fumie| lnoue, Taeko| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1996-11
Publication Title 岡大三朝分院研究報告
Volume volume67
Content Type Data or Dataset
Author Matsubara, Fumie| lnoue, Taeko| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1997-12
Publication Title 岡大三朝分院研究報告
Volume volume68
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| Mitsunobu, Yasuko| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| 光延 彰子| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| 光延 彰子| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 2001-12-01
Publication Title 岡大三朝分院研究報告
Volume volume72
Content Type Data or Dataset
Author Matsubara, Fumie| 光延 彰子| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 2002-12-01
Publication Title 岡大三朝分院研究報告
Volume volume72
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| 光延 彰子| Nagata, Takuya| Tanizaki, Yoshiro|
Published Date 2004-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Data or Dataset
Author Matsubara, Fumie| 光延 彰子| Nagata, Takuya| Tanizaki, Yoshiro|
Published Date 2003-02-01
Publication Title 岡大三朝医療センター研究報告
Volume volume73
Content Type Data or Dataset
Author Matsubara, Fumie| Inoue, Taeko| 光延 彰子| Takata, Shingo| Tanizaki, Yoshiro|
Published Date 2004-12-01
Publication Title 岡大三朝医療センター研究報告
Volume volume75
Content Type Data or Dataset
Author Matsubara, Fumie| Endou, Hiroi| Sudo, Michiyasu| Tanizaki, Yoshiro|
Published Date 1990-09
Publication Title 環境病態研報告
Volume volume61
Content Type Data or Dataset
Author Matsubara, Fumie| Endou, Hiroi| Kitani, Hikaru| Tanizaki, Yoshiro|
Published Date 1991-08
Publication Title 環境病態研報告
Volume volume62
Content Type Data or Dataset