このエントリーをはてなブックマークに追加
ID 13849
Eprint ID
13849
FullText URL
Thumnail 106_1063.pdf 3.38 MB
Title Alternative
Strongly negatively charged extracellular matrix in the mouse central nervous system
Author
Murakami, Takuro
Abstract
Neurons of the retrosplenial cortex, hippocampal subiculum, pontine nuclei, intracerebellar nuclei and some other nuclei in the mouse demonstrated a marked surface coat which was formed three or four weeks after birth. This coat was stained doubly with cationic iron colloid (pH 1.0-1.5) and aldehyde fuchsin or with alcian blue and aldehyde fuchsin. Digestion with hyaluronidase eliminated all the cationic iron colloid, aldehyde fuchsin and alcian blue stainings of the surface coat. Successive digestion with chondroitinase ABC, heparitinase and keratanase erased the cationic iron colloid and alcian blue stainings of the surface coat, but did not interfere with the aldehyde fuchsin staining of this coat. Electron microscopy of ultrathin sections revealed that the iron particles were preferentially deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans, which form an extracellular matrix in the perineuronal tissue spaces and as a charge barrier assure stable signal transmission at the axosomatic synapes with inhibitory nature. It was further observed that the neurons with such surface coats in the retrosplenial cortex and hippocampal subiculum were labeled with lectin Vicia villosa agglutinin.
Keywords
Central nervous system
neurons
strongly negatively charged extracellular matrix
sulfated proteoglycans
Note
原著
Published Date
1994-10
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue9-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1063
End Page
1071
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/106/9-10/106_9-10_1063/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma