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ID 18205
Eprint ID
18205
FullText URL
Thumnail 77_267.pdf 10.7 MB
Title Alternative
Histochemical Studies of Human Breast Tumors Part Ⅱ. On Dehydrogenases
Author
Nobuto, Hideo
Abstract
Histochemical observation of human breast tumors were caried out on lactic dehydrogenase, glucose-6-phosphate dehydrogenase, isocitric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, glutamic dehydrogenase, α-glycerophosphate dehydrogenase and β-hydroxybutyric dehydrogenase. The surgically removed breast tumors were examined; mastopathia:, fibroadenoma:, gynecomastia:, and cancer:. The tumors were cut at 20μ in -20℃ cryostat, then the sections were stained by the methods described by Pearce (60)) with Nitro-BT as the electron acceptor. The following results were obtained. Lactic dehydrogenase activity was the strongest of all dehydrogenases studied in this report. It increased in proliferation, and slightly decreased in cancer. The stromal reaction was moderate. Glucose-6-phosphate dehydrogenase activity did not decreased in cancer tissues so much as that of other dehydrogenases. These results showed that anaerobic glycolysis and hexose monophosphate shunt played an important role in breast cancer metabolism. For investigation of tricarboxylic acid cycle, succinic dehydrogenase, isocitric dehydrogenase and malic dehydrogenase were studied. These three enzymes showed similar distribution to each other. Their activity were increased in proliferation, but decreased in cancer. Their activities were especially decreased in poorly differentiated cancer cells except for scirrhous carcinoma. Glutamic dehydrogenase activity revealed a simillar pattern to that of TCA cycle enzymes. As enzymes of fatty acid metabolisum, α-glycerohosphate dehydrogenase and β-hydroxybutyric dehydrogenase were examined. The activity was relatively weak in benign tumors and very weak in cancer.
Published Date
1965-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume77
Issue
issue2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
267
End Page
281
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/77/2/77_2_267/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma