JaLCDOI 10.18926/AMO/31834
FullText URL fulltext.pdf
Author Munemasa, Mitsuru| Yoshino, Tadashi| Kobayashi, Keita| Miyake, Takayoshi| Sakugawa, Sumie Takase| Mannami, Tomohiko| Shinagawa, Katsuji| Tanimoto, Mitsune| Akagi, Tadaatsu|
Abstract <p>Reportedly, thyroid mucosa-associated lymphoid tissue (MALT) lymphoma is closely associated with Hashimoto's thyroiditis. However, it remains unknown which antigen is closely associated with thyroid MALT lymphoma. We examined whether B cell response to thyroglobulin (Tg), which is a common thyroid-specific autoantigen, is related etiologically to the pathogenesis of thyroid MALT lymphoma. Expression of human Tg antigens and Cluster of differentiation (CD) 35 was examined immunohistochemically in 15 cases of thyroid MALT lymphoma using paraffin-embedded, formalin-fixed tissue specimens. In all cases of thyroid MALT lymphoma, human Tg was detected immunohistochemically in the follicular epithelial cells and follicular dendritic cells (FDCs). These FDCs were positive by double immunostaining for anti-human Tg rabbit polyclonal antibody (Ab) and for CD35. Results showed that the Tg, a thyroid autoantigen, had immunostained the germinal center of the thyroid MALT lymphoma. The Tg was present in the FDCs, as revealed by the staining pattern of the germinal center;this fact was confirmed by double immunostaining of anti-human Tg mouse monoclonal Ab and anti-CD35 mouse monoclonal Ab. The results of our study suggest that Tg is an autoantigen that is recognized by thyroid MALT lymphoma cells.</p>
Keywords thyroglobulin follicular dendritic cells mucosa-associated lymphoid tissue lymphoma
Amo Type Original Article
Published Date 2009-04
Publication Title Acta Medica Okayama
Volume volume63
Issue issue2
Publisher Okayama University Medical School
Start Page 71
End Page 78
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19404338
Web of Sience KeyUT 000265457600001
JaLCDOI 10.18926/AMO/31574
FullText URL fulltext.pdf
Author Shinagawa, Katsuji| Imajo, Kenji| Tada, Shinya| Tsubota, Teruhiko| Kimura, Ikuro|
Abstract The activity of pulmonary lymphocytes was evaluated by the detection of interleukin-2 (IL-2) receptor alpha mRNA expression in lung cancer patients associated with diffuse interstitial shadow on roentgenograms of their lungs. Reverse transcription coupled with the polymerase chain reaction was used to detect mRNA expression. In 5 of 6 patients, IL-2R alpha mRNA expression was increased in pulmonary lymphocytes compared with 4 normal controls. The expression in this mRNA in peripheral blood lymphocytes was almost undetectable in either normal controls or these patients. These results suggest that pulmonary lymphocytes in patients with lung cancer associated with diffuse interstitial shadows are activated and may promote the inflammatory process generating pulmonary fibrosis.
Keywords pulmonary fibrosis lung cancer pulmonary lymphocytes IL-2R α mRNA RT-PCR
Amo Type Article
Published Date 1993-04
Publication Title Acta Medica Okayama
Volume volume47
Issue issue2
Publisher Okayama University Medical School
Start Page 73
End Page 78
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders Copyright© 1999 Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 8506752
Web of Sience KeyUT A1993LA45200001
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/5957
JaLCDOI 10.18926/AMO/31564
FullText URL fulltext.pdf
Author Imajo, Kenji| Shinagawa, Katsuji| Tada, Shinya| Tsubota, Teruhiko| Kimura, Ikuro|
Abstract <p>Newly designed oligonucleotide primers, KI-7 and KI-8 for the human T cell lymphotropic virus type I (HTLV-I) pX gene were synthesized using an automated DNA synthesizer. Previously known HTLV-I-infected cell lines, MT-1 and MT-2, were used as positive controls and HTLV-I-uninfected cell lines, Molt-4, SBC-3, ABC-1, and EBC-1, as negative controls. Peripheral blood mononuclear cells from 17 patients with anti-HTLV-I antibody and 10 healthy individuals without anti-HTLV-I antibody were studied by polymerase chain reaction (PCR) with KI-7 and KI-8. All DNA samples from HTLV-I-infected cell lines and 17 patients with anti-HTLV-I antibodies showed positive signals of the HTLV-I pX gene. None of the DNA samples from HTLV-I-uninfected cell lines or 10 healthy individuals showed positive signals. When serially diluted DNA of MT-2 cells were amplified by 35 cycles of PCR, the detection limit of the pX gene by using the primer pairs was DNA from about 1.5 MT-2 cells. Specificity and detectable capacity of primer pairs, KI-7 and KI-8 were confirmed to be enough to use for the diagnosis of HTLV-I infection.</p>
Keywords HTLV-1 polymerase chain reaction oligonucleotide primer DNA synthesis
Amo Type Article
Published Date 1993-12
Publication Title Acta Medica Okayama
Volume volume47
Issue issue6
Publisher Okayama University Medical School
Start Page 355
End Page 361
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8128908
Web of Sience KeyUT A1993MP00700001
JaLCDOI 10.18926/AMO/31560
FullText URL fulltext.pdf
Author Imajo, Kenji| Shinagawa, Katsuji| Tada, Shinya| Tsubota, Teruhiko| Kimura, Ikuro|
Abstract <p>The presence of the HTLV-I gene in peripheral blood mononuclear cells was studied by polymerase chain reaction in 42 patients including 16 with lung cancer, 12 with diffuse panbronchiolitis (DPB), 11 with idiopathic interstitial pneumonia (IIP), and 3 with pneumoconiosis and hematological malignancy. Sequences equal to a part of the pX gene were found in 44% of the lung cancer cases, 50% of the DPB cases, 55% of the IIP cases, and 100% of the cases of pneumoconiosis and leukemia. In the lung cancer cases, detection of the pX gene was frequently associated with the existence of diffuse interstitial pulmonary shadows. The pX gene was detected in 100% of patients with anti-HTLV-I antibody, 50% of patients with HTLV-I-related reaction and 14% of patients who tested seronegative. It may be inferred from the results that respiratory diseases that produce diffuse interstitial pulmonary shadows are closely associated with HTLV-I infection and that the HTLV-I-related reaction to the immunofluorescent test might reflect the latent infection state of HTLV-I.</p>
Keywords HTLV-1 polymerase chain reaction HTLV-I associated bronchiolo-alveolar disorder HTLV-I associated lung cancer immunofluorescent assay
Amo Type Article
Published Date 1993-12
Publication Title Acta Medica Okayama
Volume volume47
Issue issue6
Publisher Okayama University Medical School
Start Page 363
End Page 368
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8128909
Web of Sience KeyUT A1993MP00700002
Author Shinagawa, Katsuji|
Published Date 2008-08-01
Publication Title 岡山医学会雑誌
Volume volume120
Issue issue2
Content Type Journal Article
Author Shinagawa, Katsuji|
Published Date 1993-03-28
Publication Title
Content Type Thesis or Dissertation