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ID 15942
Eprint ID
15942
FullText URL
Title Alternative
Transcriptional promoter activities of recombinant proviral long terminal repeats
Author
Abstract
The transcriptional promoter activity of the proviral long terminal repeat (LTR) of a retrovirus produced in a human lymphoblastoid cell line was studied in a variety of cell types, in comparison with that of the simian virus 40 (SV40) early promoter and Rous sarcoma virus (RSV) LTR. Recombinant plasmids containing these promoter sequences linked to the chloramphenicol acetyltransferase (CAT) gene were constructed and tested in a transient assay system to measure relative rates of transcriptional activites in different cell types, including human lymphoblastoid cell lines. The transcriptional activity of this proviral LTR was much higher than that of the SV40 early promoter and RSV LTR in various cell types, especially in human lymphoblastoid cell lines. This result is consistent with the fact that this retrovirus is a lymphotropic virus. This proviral LTR could be useful for the analyses of the expression and biological activity of various genes in human lymphoblastoid cell lines.
Keywords
転写プロモーター活性
プロウイルスLTR
DNAトランスフェクション
クロラムフェニコールアセチルトランスフェラーゼ遺伝子
Published Date
1988
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume100
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
507
End Page
517
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/100/5-6/100_5-6_507/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma