Author Ohtsuki, Takashi| Miki, Shunya| Kobayashi, Shouhei| Haraguchi, Tokuko| Nakata, Eiji| Hirakawa, Kazutaka| Sumita, Kensuke| Watanabe, Kazunori| Okazaki, Shigetoshi|
Published Date 2015-12
Publication Title Scientific Reports
Volume volume5
Content Type Journal Article
Author Ohtsuki, Takashi| Kanzaki, Shigeto| Nishimura, Sae| Kunihiro, Yoshio| Sisido, Masahiko| Watanabe, Kazunori|
Published Date 2016-08
Publication Title Nature Communications
Volume volume7
Content Type Journal Article
FullText URL NatCommun_12501.pdf
Author Ohtsuki, Takashi| Kanzaki, Shigeto| Nishimura, Sae| Kunihiro, Yoshio| Sisido, Masahiko| Watanabe, Kazunori|
Abstract The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
Keywords Molecular engineering Optogenetics
Note This is an article published by Nature Publishing Group
Published Date 2016-08
Publication Title Nature Communications
Volume volume7
Start Page 12501
ISSN 2041-1723
NCID AA12645905
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version publisher
PubMed ID 27530762
DOI 10.1038/ncomms12501
Web of Sience KeyUT 000381771700001
Related Url https://doi.org/10.1038/ncomms12501