Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755


Hori, Satoshi
Tanabe, Akira
In 1948 PETERSON and JOHNSON minutely described the column partition chromatographic method for the separation and quantitative estimation of the volatile fatty acids. In this experiment the partition chromatograms we employed were prepared as follows. The column was consisted of glass tube packed with silica gel, 30 cm in length and 8mm in diameter. Water was used as stational phase and benzene, 1.2% butanol benzene, 5% butanol chloroform, 12% butanol chloroform and butanol as mobile phase. Recoveries of formic, acetic, propionic and butyric acid were 104%, 101%, 99% and 100% respectively. We tried in vain to estimate the valeric acid in the presence of the acids previously described.