Studies on Glucose Metabolism of Leukemic Cell Part 2. The Respiration and Glycolysis of the Spleen in Normal, Preleukemic and Leukemic Mice of DBA Strain

The respiration and glycolysis of the spleen in 10 normal mice, 17 mice of preleukemic stage and 14 induced lymphatic leukemia mice by painting with methylcholanthrene, was studied. In addition, the effect of glucose on the rates of the oxygen consumption was studied. 1) In 10 normal mice of strain DBA, Q(O(2)) was 7.75, Q(O(2))(M), 1.56 and Q(N(2))(M), 5.90. 2) The rate of the oxygen consumption was a little greater in the mice of the preleukemic stage than in normal mice, but there was no difference statistifically between both groups. The rates of the aerobic and anaerobic glycolysis were higher in mice of the preleukemic stage than in normal mice statistifically. The percentages of lymphoblast in the spleen was higher in the mice of the preleukemic stage than in normal mice. 3) The rates of the oxygen consumption, aerobic glycolysis and anaerobic glycolysis were higher in the induced lymphathic leukemia mice than in normal and preleukemic mice. There was a parallel relation between the percentage of lymphoblast in the spleen, and the oxygen consumption and anaerobic glycolysis of the spleen in induced lymphatic leukemia. 4) The oxygen consumption rate of the spleen of all mice with induced lymphatic leukemia was decreased by addition of glucose in a concentration of 0.2 p.c. in Ringer's solution. Namely, Crabtree's effect was found in the spleen with the induced lymphatic leukemia. On the other hand, there is no Crabtree's effect in the spleen of normal and preleukemic mice.