As a step for the measurement of the reticuloendothelial systems (RES) functions, there is a method of labeling serum iron colloid with 59Fe. Employing the same method, this study was conducted in an attempt to see whether there would be any differences in the radiation effects when 59Fe is injected at varying doses.
Experimentals: The animals used were healthy normal rabbits and they were divided into different groups of those receiveng the doses of 10μCi, 50μCi, 250μCi, and 700μCi as well as those injectcd with non-radioactive serum iron colloid solution, and simultaneously congored was injected to every group and then the congored indices were obtained. One week later identically the same doses for the respective groups were again injected. and also congored, and the congored indices of these two trials were compared. Fluctuations of the 59Fe level in whole blood were observed. The animals were sacrificed by exsanguination at 12 hours and 96 hours after the injection. On the tenth day after the injection hemograms were studied to determine the effects of the injections.
Results: 1. It has been found that the higher the dose of 59Fe, the greater is the inhibitory effect on the 59Fe incorporation into hemoglobin, but the dose of 59Fe itself seems to have little effect on the function of phagocytosis.
2. There can be observed no appreciable differences in the hemograms on the tenth day after the injection among these groups of animals.
3. The quantity of iron deposited in various organs at 12 hours and 96 hours after the injection decreased in the order of bone marrow, liver and spleen in all the groups receiving 59Fe but there could be seen no differences among these various groups.