Vital Observations on the Movement of the Specific Granules of Granulocytes in Bone-Marrow Tissue Culture Part 1. A Study on Pseudo-eosinophils and Eosinophils of Normal Rabbits

By means of bone-marrow tissue cultnre the author studies the movement of the specific granules of pseudo-eosinophils and eosinophils in normal rabbits, and obtained the following results: 1. The movement cycles of these cells are classified into the original stage, the preparatory stage, the pseudopodia formation stage, the migratory stage of the main body, the stage of caudal retraction, and the stage of distribution of granules. 2. The types of the granular movement are classified into the molecular movement, linear formation advance, advance line after line, and semi-radial linear forward movement, zigzag forward movement, disproportionate linear forwad movement with semi-crescent front, circular movement, reverse-flow movement, alternate movement, individual movement, and irregular movement. and in addition, are included the rapid advance immediately after the nucleus advance and the rapid forward movement after passing through holes as the behaviors of the granular movements. However, the main granular movement is the linear formation advance. 3. The molecular and irregular movements can hardly be observed in eosinophils, but the advance line by iine, semiradial linear forward movement, and zigzag forward movement can be observed in eosinophils at the time of semi-radial movement of the cell. The reverseflow mevement may be observed either at pseudopodia or at the tail end, and as an atypical one, a movement in opposite direction may be observed. There is no marked difference in the rate of appearance of various granular movements from the duration of tissue culture. 4. The granular movement is not automatic, but it is the movement of granules riding on the current of cytoplasma. 5. The molecular movement is not necessarily involved in the negativity of the cell movement, but it seems to indicate the potential motive power.