Studies on Reticulocyte Ripening Substances Part 1. The Action of Bovine Liver Extract on the Maturation of Reticulocytes in Rabbits

According to the method deviced by Seno and orthers the mode of maturation of reticulocytes of anemic rabbits have been observed in vitro with or without addition of the emulsion of bovine liver. The observations prove that the supernatant of the liver emulsion has the ability to accelerate the maturation of the reticulocytes showing complete maturation by about two hours in the oxygenated blood where the supernatant is added, while it takes about 9 hours under the same condition without addition of the suppernatant. Intravenous introduction of a large dose of the supernatant into the anemic rabbits results in a temporary and rapid derease of reticulocytes in circulating blood. These observations indicate that the ripening substances like that in guinea pig liver found by Seno and others likewise exist in the bovine liver.