Studies on the Combination of Bilirubin with Proteins Part 1. Combination of Bilirubin with Albumin and with Globulin

With the purpose to study the combinations of bilirubin with albumin and with globulin using a veromal buffer solution (pH 8.5) as the elctrolyte the author estimated the absorption curve by means of paper electrophoresis and by paper chromatography at the first dimesnion using 2% cane sugar solution as the developer and with D-K type Beckman's autospectro-photometer; and obtained the following results. 1. Sodium bilirubinate that is a direct bilirubin and dibasic acid bilirubin which is an indirect bilirubin both combine with albumin. 2. The mode how bilirubin combines with albumin can not be the controlling factor of the direct or the indirect bilirubin reaction. 3. When desalting is done on the combined solution of sodium bilirubinate and albumin, a mixed solution of bilirubin and albumin is obtained, but no bilirubin can be isolated. 4. It is quite difficult to combine blirubin with globulin.