With the use of various stains the author studied Donaggio reaction of urine and obtained the following results. 1. Methylene blue (MB), thionine (TN), crystal violet (CV), fuchsin (FS), and thymol blue (TB) are all applicable for Donaggio reaction. 2. The sensitivity of various stains is in the descending order of TN > FS > CV > TB > MB. 3. MB, TB, and TN are sensitive stains that respond ralatively rapidly to the reaction, whereas CV and FS are the stains that respond to the reaction rather slowly. 4. MB, TN and TB seem to be especially useful stains for the determination of the grade of fatigue by Donaggio reaction with the urine both before and after work.