Using rabbits, guinea pigs, and mice as the test animals, the author studied the production of homologous-liver-antibody and auto-liver-antibody by preparing various organ antigens of these animals and injecting a hypertonic physiological saline solution of these antigens into the liver of respective animals asceptically so as to avoid the sensitization of homologous animals; and obtained the following results. 1. It is possible to produce auto-liver-antibody by injecting homologous-liver-antigen and auto-liver-antigen. 2. These antibodies can combine with homologous- and auto-liver-antigens in vivo. 3. The homologous-liver-antigens and autoantigens have been found to have a specificity to organs, but this is just a matter of comparison. Of the two the specifity of the latter is more marked.