Journal of Okayama Medical Association
Published by Okayama Medical Association

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Studies on the role of eosinophils in the pathogenesis of bronchial asthma Part 1. Evaluation of serum eosinophil chemotactic factor in asthmatic responses using eosinophils purified by flow cytometry

Kawada, Ichiro
102_733.pdf 671 KB
Published Date
1990-06
Abstract
The serum eosinophil chemotactic factor (ECF) was evaluated by use of the modified Boyden chamber method. Flow cytometry (FCM) was applied to obtain highly purified eosinophils from peripheral blood of normal subjects. The maximal chemotaxis of these eosinophils was shown at 1×10(-7) M of platelet activating factor and leukotriene B4. In bronchial asthmatics with immediate asthmatic response (IAR), serum ECF activity showed only an early rise, and that in asthmatics with late asthmatic response (LAR) showed a precedent rise before bronchial reaction. Asthmatic patients with both IAR and LAR showed biphasic elevation in ECF activity. These findings suggest that the function of ECF in the pathogenesis of IAR is different from that in LAR. The eosinophils purified by FCM were very useful for measurement of ECF by the Boyden chamber method.
Keywords
Bronchial asthma
Eosinophil
Eosinophil chemotactic factor (ECF)
Flow cytometry
ISSN
0030-1558
NCID
AN00032489