Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

本邦におけるキニノゲン欠損症の分子生物学的研究 第1編 キニノゲンに対するモノクローナル抗体による各ドメインの検索

Ishimaru, Eiji
Thumnail 102_365.pdf 4.59 MB
Abstract
Analysis of kininogen molecule status in four Japanese families affected with total kininogen deficiency and one Japanese family with isolated high molecular weight kininogen deficiency was performed using an immuoblotting method with monoclonal antibodies to high molecular weight kininogen (HKG H 12, L 7, L 17). The purified high molecular weight kininogen showed two bands with apparent mot wts of 120, 000 and 105, 000, respectively, and the purified low molecular weight kininogen showed a single band with an apparent mot wt of 60, 000. In normal human plasma, these three bands were also detected. No variant molecule of the kininogens was present in either cases of total kininogen deficiency or isolated high molecular weight kininogen deficiency.
Keywords
high molecular weight kininogen
low molecular weight kininogen
kininogen deficiency
immunoblotting
monoclonal antibody
ISSN
0030-1558
NCID
AN00032489