Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

Human serum lipoprotein-cyclodextrin interaction in agarose gel

Ichimura, Mitsuko
Tohge, Hiroko
Sakiyama, Junko
Mori, Shuji
Ito, Sekiko
Nakata, Yasunari
Endo, Hiroshi
Cyclodextrins (CDs) are cyclic oligosaccharides with low molecular weight. They have been known to bind to some serum lipoproteins and to form complexes. To elucidate whether each serum lipoprotein subclass could be separated by electrophoresis using CDs, we performed preliminary experiment, in which lipoprotein-CDs interaction was examined on electrophoresis with agarose gel. When the supporting agarose gel containing both α-CD and β-CD was prepared and was applied to isoelectric focusing for fractionating serum lipoproteins, apoB lipoproteins were found to be clearly separated into several fractions on this electrophoresis. This finding suggested that apoB lipoprotein may be detected as isolated form by arranging amounts of added CDs.
血清apoB含有リポタンパク質 (serum apoB lipoprotein)
シクロデキストリン (cyclodextrin)
等電点電気泳動 (isoelectric focusing (IEF))
agarose gel