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ID 910
Eprint ID
910
FullText URL
Title Alternative
胚珠培養、胚培養による3倍体マスカット個体の育成
Author
Okamoto, Goro
Tanakamaru, Naomi
Omori, Naoki
Abstract
To breed new seedless grape cultivars we crossed 'Muscat of Alexandria' (2x) and its 4x mutant reciprocally and cultured the immature ovules and embryos in vitro. Ovules sampled 50 days after crossing could develop embryos on a liquid medium of half strength of MS supplemented with 2 ppm of IAA, 0.4 ppm of GA3 and 0.1 % of activated charcoal. After 55 days culture, normally developed embryos were obtained from 20 to 30 % of the ovules from 4x×2x and 50 to 70 % of those from 2x×4x. Embryos taken out from the cultured ovules were planted on an agar medium of MS or N&N for 55 to 65 days. Ten to twenty % of embryos from 2x×4x crossing grew to rooted plantlets successfully. They were proved to be triploid after the measurement of chromosome number. Embryos from 4x×x2x, however, could not grow to plantlets because of the failure of rooting.
Keywords
胚珠培養
胚培養
マスカット個体
育成
Published Date
1993
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific Reports of the Faculty of Agriculture Okayama University
Volume
volume82
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture, Okayama University
Start Page
25
End Page
29
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa