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ID 61413
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Author
Mukudai, Yoshiki Biodental Research Center, Okayama University Dental School
Abstract
CCN2/CTGF is a multifunctional growth factor. Our previous studies have revealed that CCN2 plays important roles in both growth and differentiation of chondrocytes and that the 3′-untranslated region (3′-UTR) of ccn2 mRNA contains a cis-repressive element of gene expression. In the present study, we found that the stability of chicken ccn2 mRNA is regulated in a differentiation stage-dependent manner in chondrocytes. We also found that stimulation by bone morphogenetic protein 2, platelet-derived growth factor, and CCN2 stabilized ccn2 mRNA in proliferating chondrocytes but that it destabilized the mRNA in prehypertrophic-hypertrophic chondrocytes. The results of a reporter gene assay revealed that the minimal repressive cis-element of the 3′-UTR of chicken ccn2 mRNA was located within the area between 100 and 150 bases from the polyadenylation tail. Moreover, the stability of ccn2 mRNA was correlated with the interaction between this cis-element and a putative 40-kDa trans-factor in nuclei and cytoplasm. In fact, the binding between them was prominent in proliferating chondrocytes and attenuated in (pre)hypertrophic chondrocytes. Stimulation by the growth factors repressed the binding in proliferating chondrocytes; however, it enhanced it in (pre)hypertrophic chondrocytes. Therefore, gene expression of ccn2 mRNA during endochondral ossification is properly regulated, at least in part, by changing the stability of the mRNA, which arises from the interaction between the RNA cis-element and putative trans-factor.
Published Date
2005-02-04
Publication Title
Journal of Biological Chemistry
Volume
volume280
Issue
issue5
Publisher
American Society for Biochemistry and Molecular Biology
Start Page
3166
End Page
3177
ISSN
0021-9258
NCID
AA00251083
Content Type
Journal Article
Related Url
isVersionOf https://doi.org/10.1074/jbc.M411632200
language
英語
Copyright Holders
© 2005 ASBMB.
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publisher
DOI
PubMed ID
Web of Science KeyUT
Funder Name
Japan Society for the Promotion of Science
License
https://creativecommons.org/licenses/by/4.0/