ID 61263
FullText URL
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Author
Hirano, Minako Bio Photonics Laboratory, The Graduate School for the Creation of New Photonics Industries
Yamamoto, Daiki Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Asakura, Mami Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hayakawa, Tohru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Mise, Shintaro Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
Matsumoto, Akinobu Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
Ide, Toru Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Abstract
Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, alpha-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.
Keywords
ion channel
lipid bilayer
single-channel recording
Published Date
2020-12-01
Publication Title
Micromachines
Volume
volume11
Issue
issue12
Publisher
MDPI
Start Page
1070
ISSN
2072-666X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 by the authors.
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3390/mi11121070
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
15K13725
18K06157