ID 60979
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Janssen, Jan P. Department of Nuclear Medicine, University Hospital Würzburg
Hoffmann, Jan V. Department of Nuclear Medicine, University Hospital Würzburg
Kanno, Takayuki Comprehensive Heart Failure Centre, University Hospital Würzburg
Nose, Naoko Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Grunz, Jan-Peter Department of Diagnostic and Interventional Radiology, University Hospital Würzburg
Onoguchi, Masahisa Department of Quantum Medical Technology, Graduate School of Medical Sciences, Kanazawa University
Chen, Xinyu Comprehensive Heart Failure Centre, University Hospital Würzburg
Lapa, Constantin Nuclear Medicine, Medical Faculty, University of Augsburg
Buck, Andreas K. Department of Nuclear Medicine, University Hospital Würzburg
Higuchi, Takahiro Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Abstract
We aimed to investigate the image quality of the U-SPECT5/CT E-Class a micro single-photon emission computed tomography (SPECT) system with two large stationary detectors for visualization of rat hearts and bones using clinically available Tc-99m-labelled tracers. Sensitivity, spatial resolution, uniformity and contrast-to-noise ratio (CNR) of the small-animal SPECT scanner were investigated in phantom studies using an ultra-high-resolution rat and mouse multi-pinhole collimator (UHR-RM). Point source, hot-rod, and uniform phantoms with Tc-99m-solution were scanned for high-count performance assessment and count levels equal to animal scans, respectively. Reconstruction was performed using the similarity-regulated ordered-subsets expectation maximization (SROSEM) algorithm with Gaussian smoothing. Rats were injected with similar to 100 MBq [Tc-99m]Tc-MIBI or similar to 150 MBq [Tc-99m]Tc-HMDP and received multi-frame micro-SPECT imaging after tracer distribution. Animal scans were reconstructed for three different acquisition times and post-processed with different sized Gaussian filters. Following reconstruction, CNR was calculated and image quality evaluated by three independent readers on a five-point scale from 1="very poor" to 5="very good". Point source sensitivity was 567 cps/MBq and radioactive rods as small as 1.2 mm were resolved with the UHR-RM collimator. Collimator-dependent uniformity was 55.5%. Phantom CNR improved with increasing rod size, filter size and activity concentration. Left ventricle and bone structures were successfully visualized in rat experiments. Image quality was strongly affected by the extent of post-filtering, whereas scan time did not have substantial influence on visual assessment. Good image quality was achieved for resolution range greater than 1.8 mm in bone and 2.8 mm in heart. The recently introduced small animal SPECT system with two stationary detectors and UHR-RM collimator is capable to provide excellent image quality in heart and bone scans in a rat using standardized reconstruction parameters and appropriate post-filtering. However, there are still challenges in achieving maximum system resolution in the sub-millimeter range with in vivo settings under limited injection dose and acquisition time.
Keywords
Biomarkers
Diagnostic markers
Medical research
Preclinical research
Translational research
Published Date
2020-10-29
Publication Title
Scientific Reports
Volume
volume10
Issue
issue1
Publisher
Nature Research
Start Page
18616
ISSN
2045-2322
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2020
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isVersionOf https://doi.org/10.1038/s41598-020-75696-0
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