ID 58261
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Hirano, Yutaka Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sugimoto, Seiichiro Department of General Thoracic Surgery, Okayama University Hospital ORCID Kaken ID publons researchmap
Yamamoto, Sumiharu Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Okada, Masanori Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Otani, Shinji Department of Organ Transplant Center, Okayama University Hospital
Ohara, Toshiaki Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Yamane, Masaomi Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID researchmap
Matsukawa, Akihiro Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Oto, Takahiro Department of Organ Transplant Center, Okayama University Hospital Kaken ID publons
Toyooka, Shinichi Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Abstract
Objective: In lung transplantation (LTx) from donation after cardiac death (DCD), the donor lungs are inevitably exposed to warm ischemic time (WIT) between the cardiac arrest and the initiation of cold preservation. We conducted this study to examine the effect of prolonged WIT on lung allograft rejection in a murine model of LTx from DCD.
Methods: Allogeneic BALB/c → B6 LTx from DCD was performed with a WIT of 15 min (WIT15 group, n = 5) or 60 min (WIT60 group, n = 5). Recipients were immunosuppressed by perioperative costimulatory blockade. The lung allografts were analyzed by histology and flow cytometry on day 7 after the LTx.
Results: Histologically, the rejection grade in the WIT60 group was significantly higher than that in the WIT15 group (3.4 ± 0.4 vs. 2.2 ± 0.2, P = 0.0278). Moreover, the intragraft CD8+ to CD4+ T cell ratio in the WIT60 group was significantly higher than that in the WIT15 group (2.3 ± 0.12 vs. 1.2 ± 0.11, P < 0.0001).
Conclusions: Prolonged WIT could exacerbate the severity of lung allograft rejection after LTx from DCD. Minimization of the WIT could improve the outcomes after LTx from DCD.
Keywords
Brain dead donor
Donation after cardiac death
Lung transplantation
Organ preservation
Rejection
Note
This is a post-peer-review, pre-copyedit version of an article published in General Thoracic and Cardiovascular Surgery. The final authenticated version is available online at: http://dx.doi.org/10.1007/s11748-019-01181-9.
Published Date
2019-07-31
Publication Title
General Thoracic and Cardiovascular Surgery
Volume
volume68
Issue
issue1
Publisher
Springer
Start Page
57
End Page
62
ISSN
1863-6705
NCID
AA12201344
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
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DOI
Web of Science KeyUT
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isVersionOf https://doi.org/10.1007/s11748-019-01181-9
License
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
Citation
Hirano, Y., Sugimoto, S., Yamamoto, S. et al. Prolonged warm ischemia exacerbated acute rejection after lung transplantation from donation after cardiac death in a mouse. Gen Thorac Cardiovasc Surg 68, 57–62 (2020). https://doi.org/10.1007/s11748-019-01181-9
Funder Name
Japan Society for the Promotion of Science
助成番号
24791461