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Zahra, Maram Hussein Division of Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University
Salem, Tarek A. R. Department of Biochemistry, College of Medicine, Qassim University
El-Aarag, Bishoy Division of Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University ORCID
Yosri, Nermeen Department of Chemistry, Faculty of Science, Menoufia University
EL-Ghlban, Samah Biochemistry Division, Chemistry Department, Faculty of Science, Menoufia University
Zaki, Kholoud Department of Chemistry, Faculty of Science, Menoufia University
Marei, Amel H. Department of Chemistry, Faculty of Science, Menoufia University
Abd El-Wahed, Aida Department of Chemistry, Faculty of Science, Menoufia University
Saeed, Aamer Department of Chemistry, Quaid-i-Azam University
Khatib, Alfi Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia
AlAjmi, Mohamed F. Pharmacognosy Group, College of Pharmacy, King Saud University
Shathili, Abdulrahman M. Al-Rayan Research and Innovation Center, Al-Rayan Colleges
Xiao, Jianbo Institute of Chinese Medical Sciences, University of Macau
Khalifa, Shaden A. M. Clinical Research Centre, Karolinska University Hospital
El-Seedi, Hesham R. Department of Chemistry, Faculty of Science, Menoufia University
BACKGROUND/AIM:Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers.
METHODS:The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model.
RESULTS:A. zerumbet extracts, specially CH2Cl2 and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH2Cl2 extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, 1H-NMR and 13C-NMR. A. zerumbet extracts, specially MeOH and CH2Cl2 extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC50 values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively.
CONCLUSION:Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.
Ehrlich ascites carcinoma
© 2019 by the authors.
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