ID | 58056 |
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Jinno, Katsuya
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hiramatsu, Bunta
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Tsunashima, Kenta
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Fujimoto, Kayo
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Sakai, Kenji
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Kiwa, Toshihiko
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Tsukada, Keiji
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Abstract | Liquid-phase magnetic immunoassay (MIA) using magnetic nano-particles (MNPs) has been studied as a more rapid method compared to optical methods for inspecting proteins and viruses. MIA can estimate the number of conjugated antibodies without being washed differently from conventional optical immunoassay. However, in the case of the liquid phase, it is considered that the magnetic properties of MNPs are affected by physical properties such as viscosity and impurity substances such as biological substances contained in the blood. In this study, the effect of sodium chloride (NaCl) in buffer and serum solution was evaluated to reveal the effect of serum because the sodium (Na+) and chloride (Cl-) ions in the serum dominate ion balance of blood. The measurement results of AC magnetic susceptibility and a dynamic light scattering (DLS) showed that the aggregation of MNPs was largely affected by the concentration of NaCl. This effect of the NaCl could be explained by shielding of the surface charge of MNPs by ions in the solution. Although the concentrations of NaCl in the buffer and serum solution were almost same, we found that MNPs were aggregated more in their size for those in the serum solution because of other impurities, such as proteins. These results suggest evaluation of effects of the contaminants in serum and optimization of polymer coatings of MNPs could be important factors to realize measurements of magnetic immunoassay with high accuracy. (C) 2019 Author(s).
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Published Date | 2019-12-20
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Publication Title |
AIP Advances
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Volume | volume9
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Issue | issue12
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Publisher | American Institute of Physics
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Start Page | 125317
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ISSN | 2158-3226
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © Author(s) 2019
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File Version | publisher
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DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1063/1.5130168
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License | http://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP15H05764
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