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ID 57901
Sort Key
3
Title Alternative
Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
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Author
Kajiyama, Yuki Graduate School of Environmental and Life Science, Okayama University
Mizobata, Satsuki Graduate School of Environmental and Life Science, Okayama University
Akaji, Shusaku Graduate School of Environmental and Life Science, Okayama University
Nemoto, Michiko Graduate School of Environmental and Life Science, Okayama University
Tamura, Takashi Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Inagaki, Kenji Graduate School of Environmental and Life Science, Okayama University Kaken ID researchmap
Abstract
 Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
Abstract Alternative
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Keywords
glycine oxidase
Marinomonas mediterranea
cysteine tryptophilquinone
recombinant expression
enzymatic glycine assay
Note
原著論文 (Original paper)
Publication Title
Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date
2020-02-01
Volume
volume109
Publisher
岡山大学農学部
Publisher Alternative
The Faculty of Agriculture, Okayama University
Start Page
1
End Page
6
ISSN
2186-7755
Content Type
Departmental Bulletin Paper
OAI-PMH Set
岡山大学
language
日本語
File Version
publisher
Eprints Journal Name
srfa