ID 57810
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Matsuura, Yuki Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Ueda, Masashi Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University ORCID Kakenhi
Higaki, Yusuke Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University Kakenhi
Watanabe, Keiko Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Habara, Shogo Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
Kamino, Shinichiro Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University ORCID Kakenhi
Saji, Hideo Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University
Enomoto, Shuichi Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University Kakenhi
Abstract
INTRODUCTION:
Nicotinic acetylcholine receptors (nAChRs) are of great interest because they are implicated in higher brain functions. Nuclear medical imaging is one of the useful techniques for noninvasive evaluation of physiological and pathological function in living subjects. Recent progress in nuclear medical imaging modalities enables the clear visualization of the organs of small rodents. Thus, translational research using nuclear medical imaging in transgenic mice has become possible and helps to elucidate human disease pathology. However, imaging of α4β2 nAChRs in the mouse brain has not yet been performed. The purpose of this study was to assess the feasibility of single-photon emission computed tomography (SPECT) with 5-[(123)I]iodo-3-[2(S)-azetidinylmethoxy]pyridine ([(123)I]5IA) for evaluating α4β2 nAChR availability in the mouse brain.
METHODS:
A 60-min dynamic SPECT imaging session of α4β2 nAChRs in the mouse brain was performed. The regional distribution of radioactivity in the SPECT images was compared to the density of α4β2 nAChRs measured in an identical mouse. Alteration of nAChR density in the brains of Tg2576 mice was also evaluated.
RESULTS:
The mouse brain was clearly visualized by [(123)I]5IA-SPECT and probe accumulation was significantly inhibited by pretreatment with (-)-nicotine. The regional distribution of radioactivity in SPECT images showed a significant positive correlation with α4β2 nAChR density measured in an identical mouse brain. Moreover, [(123)I]5IA-SPECT was able to detect the up-regulation of α4β2 nAChRs in the brains of Tg2576 transgenic mice.
CONCLUSIONS:
[(123)I]5IA-SPECT imaging would be a promising tool for evaluating α4β2 nAChR availability in the mouse brain and may be useful in translational research focused on nAChR-related diseases.
Keywords
5-[(123)I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([(123)I]5IA)
Alzheimer's disease
Mouse
Nicotinic acetylcholine receptor (nAChR)
Single-photon emission computed tomography (SPECT)
Tg2576
Published Date
2016-06
Publication Title
Nuclear Medicine and Biology
Volume
volume43
Issue
issue6
Publisher
Elsevier Science
Start Page
372
End Page
378
ISSN
0969-8051
NCID
AA10864894
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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Related Url
isVersionOf https://doi.org/10.1016/j.nucmedbio.2016.02.007
Citation
Yuki Matsuura, Masashi Ueda, Yusuke Higaki, Keiko Watanabe, Shogo Habara, Shinichiro Kamino, Hideo Saji, Shuichi Enomoto, Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [123I]5IA, Nuclear Medicine and Biology, Volume 43, Issue 6, 2016, Pages 372-378, ISSN 0969-8051, https://doi.org/10.1016/j.nucmedbio.2016.02.007.
Funder Name
Japan Society for the Promotion of Science
助成番号
26670562