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ID 52889
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Author
Tsuchiya, Hiroki
Abstract
Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury.
Keywords
Capsaicin
Behaviour
c-Fos
Medullary dorsal horn
Immunohistochemistry
Nerve injury
Published Date
2014-12
Publication Title
Archives of Oral Biology
Volume
volume59
Issue
issue12
Publisher
Pergamon-Elsevier Science Ltd.
Start Page
1334
End Page
1341
ISSN
0003-9969
NCID
AA00548457
Content Type
Journal Article
language
英語
Copyright Holders
© 2014 Elsevier Ltd. All rights reserved.
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author
Refereed
True
DOI