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ID 46908
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Author
Shukunami, Chisa
Amemiya-Kudo, Michiyo
Shimano, Hitoshi
Abstract
During musculoskeletal development, Sry-type HMG box 9 (Sox9) has a crucial role in mesenchymal condensation and chondrogenesis. On the other hand, a tissue-specific basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) regulates the differentiation of tendon and ligament progenitors. Whereas these two transcription factors cooperatively participate in the determination of cellular lineages, the precise interaction between Sox9 and Scx remains unclear. We have previously demonstrated that the Sox9-dependent transcription is synergistically activated by several Sox9-associating molecules, such as p300 and Smad3, on chromatin. In this study, we investigated the function of Scx in the Sox9-dependent transcription. The expression of α1(II) collagen (Col2a1) gene was stimulated by an appropriate transduction of Sox9 and Scx. Scx and its partner E47, which dimerizes with other bHLH proteins, cooperatively enhanced the Sox9-dependent transcription in luciferase reporter assays. Coactivator p300 synergistically increased the activity of Sox9-regulated reporter gene, which contains promoter and enhancer regions of Col2a1, in the presence of Scx and E47. Immunoprecipitation analyses revealed that Scx and E47 formed a transcriptional complex with Sox9 and p300. Scx/E47 heterodimer also associated with a conserved E-box sequence (CAGGTG) in the Col2a1 promoter on chromatin. These findings suggest that Scx and E47 might modulate the primary chondrogenesis by associating with the Sox9-related transcriptional complex, and by binding to the conserved E-box on Col2a1 promoter.
Keywords
E47
p300
Scleraxis
Sox9
Transcription
Published Date
2010-01
Publication Title
The International Journal of Biochemistry & Cell Biology
Volume
volume42
Issue
issue1
Publisher
Pergamon-Elsevier Science Ltd.
Start Page
148
End Page
156
ISSN
1357-2725
NCID
AA11036038
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2009 Elsevier Ltd. All rights reserved.
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Refereed
True
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