このエントリーをはてなブックマークに追加
ID 40723
FullText URL
Thumnail 101_167.pdf 2.29 MB
Title Alternative
Studies on urinary protein after physical load Report 3. New micro determination of urinary protein with CBB stained agar plate (with specific reference to urinary protein before and after physical exercise)
Author
Miyai, Taizo
Abstract
A new micro determination of urinary protein using Coomassie Brilliant Blue G-250 (CBB) and sulfosalicyl acid containing agar plates was devised. The correlation between the concentration of urinary protein using CBB colorimetry and that using our staining method on agar plate was determined. 1. Assay procedure 1.5g of agar was disselved 90ml of water while waring at 60°C (solution A) 0.005g CBB was disselved into 5ml of water (solution B) solution A and solution B and were mixed and wate was added to a final volume of 100ml. For urinary protein determination, 15μl of urine were spotted on agar plate. After 15 minutes, colored spots were evaluated. For the semiquantitative determination, the plate was dried at 37°C for 30 minutes and spots were compared wdth a standard spot using the naked eye. For the quantitative determination, the plate was dried at 37°C for 30 minutes and spots were analyzed with a densitometer. 2. The newly devised CBB method is convenient for micro assay of urinary protein. The urine samples can be determined in small amounts and the method is simple highly sensitive and highly accurate. Many urine specimens can be measured in a field survey. Result can be stored for long periods of time after blotting into writing menbrane-filter. 3. This method is in the range of 1.2%4.5% in producing good date, tab proside piad has excellent reproducibility an average recovery of 98.8±4.9%.
Keywords
尿微量蛋白質定量
gel spot色素法
運動尿
Published Date
1989-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume101
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
167
End Page
174
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/101/1-2/101_1-2_167/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma