ID 32865
JaLCDOI
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Author
Murai, Hiroyasu
Hiragami, Fukumi
Kawamura, Kenji
Motoda, Hirotoshi
Koike, Yoshihisa
Inoue, Shigeki
Kumagishi, Kanae
Kano, Yoshio
Abstract

Among the 3 mitogen-activated protein kinases -- ERK, p38 MAPK and JNK -- JNK has been suggested to participate in apoptosis, whereas p38 MAPK is thought to be part of the differentiation response. There are many common inducers of JNK and p38 MAPK, but the mechanisms underlying the differential response to apoptosis and differentiation are poorly understood. We found that heatshock activated p38 MAPK at 3min after exposure to a temperature of 44 in stress-hypersensitive PC12m3 mutant cells, while it activated JNK at 20min after the same heat treatment. However, heat shock activated p38 MAPK 5min after heat treatment and JNK 10min after heat treatment in PC12 parental cells. The extent of phosphorylation of p38 MAPK induced by heat shock in PC12m3 cells was significantly greater than that in PC12 parental cells, and a high level of heat-shock-induced neurite outgrowth was observed only in PC12m3 cells. On the other hand, heat-shock-induced JNK activation appeared more quickly and apoptosis started earlier in PC12 parental cells. These findings indicate that short stress induces p38 MAPK and longer stress induces JNK, and that the response of these kinases to heat shock differs depending on cell type.

Keywords
heat shock
neurite outgrowth
p38 MAP kinase
JNK
PC12 mutant cells
Amo Type
Original Article
Published Date
2010-02
Publication Title
Acta Medica Okayama
Volume
volume64
Issue
issue1
Publisher
Okayama University Medical School
Start Page
55
End Page
62
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT