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ID 19763
Eprint ID
19763
FullText URL
Thumnail 68_1297.pdf 448 KB
Title Alternative
Studies on Parathion-Splitting Enzyme. Part 1. Parathion-splitting activity of blood serum with special reference to its estimation
Author
Shiigi, Teiji
Abstract
During the course ofour study on parathion (organophosphorus insecticide) poisoning we happened on the discovery that the human blood serum promoted the decomposition of this insecticide. A procedure for the estimation of the promoting activity of blood serum in splitting parathion was developed to examine this phenomenon quantitatively. In this procedure, the mixture of serum, emulsion of parathion and bicarbonate-carbonate buffer of pH 10 in a fixed proportion was incubated at 37℃ for an hour, and the amount of paranitrophenol liberated from parathion was measured by colorimetry to express the splitting activity in terms of γ of paranitrophenol per ml of serum. A new enzyme that had not hitherto been described was supposed to be responsible for the parathion-splitting activity of serum after careful examination of its property with special reference to the ambient conditions (pH of the medium, temperature, amount of substrate used, inhibitor, etc.). The enzyme was quite different from alkaline phosphatase, cholinesterase and A-esterase (E-600 esterase) which had been believed to be concerned with the detoxication of parathion. Normal range of parathion splitting activity of human serum by the proposed method of the author was 134 ± 45 γ/ml for males and 180 ± 62 γ/ml for females.
Published Date
1956-09-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume68
Issue
issue9
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1297
End Page
1303
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/68/9/68_9_1297/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma