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ID 18697
Eprint ID
18697
FullText URL
72_1783.pdf 320 KB
Title Alternative
Action of Various Anti-Carcinogenic Agents on the Egg Division in Sea-Urchin Egg
Author
Yamamoto, Michio
Utsumi, Kozo
Ohara, Sachiko
Yamamoto, Goki
Seno, Satimaru
Ueno, Hisako
Kobayashi, Junichi
Abstract
Using various anti-carcinogenic agents (nitrogen mustard N-oxyd as an alkylating agent, 8-azaguanine as an anti-metabolite, and sarcomycin-Na, carzinophilin, mitomycin C, chromycin, actinomycin and sanamycin as inhibitory anti-carcinogenic agents) the authors studied the actions of these agents on the spermatozoa and eggs of sea-urchin by observing the rates of the membrane formation at insemination and egg division and obtaind the following results. 1. When treated with the solution of various anti carcinogenic agents diluted ten-fold the optimal qunatity for the human body for ten minutes, the spermatozoa of sea-urchin (Heliocidaris crassistina) are hardly affected by these agents. 2. In the cases where sea-urchin eggs (Hemicentrotus Pulcherrimus) are treated for 30 minutes with different anti-carcinogenic agents at various concentrations, the higher the concentration, the greater is the decrease in the rates of the membrane formation and egg division at insemination of normal spermatozoa, and in the case treated with the solution diluted 50-fold of the optimal quantity for the human body the inhibitory action of the agents increases in the ascending order of: carzinophilin > Thio TEPA > N itrogen mustard > mytomycin > Sanamycin > chromomycin > actinomycin. 3. When treated with the 50-fold solution these agents as compared with the proximal concentration for the human body, at the intervals of 20, 40, 60. 80, and 90 minutes after the fertilization, mytomycin and carzinophilin markedly inhibit the fusion of male and female nuclei while chromomycin, sarcomycin and nitrogen mustard suppress the cell division at metaphase, and chromomycin has especially strong inhibitory action on the cell. 4. When the effects of these agents are compared with those of OX-substance with respect to their concentration, it has been clarified that the action of these agents on sea-urchin eggs and spermatozoa are weaker than that of OX substance.
Published Date
1960-10-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume72
Issue
issue8-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1783
End Page
1787
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/72/8-10/72_8-10_1783/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma