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ID 18260
Eprint ID
18260
FullText URL
76_215.pdf 478 KB
Title Alternative
On the Properties of a Cell Strain, JTC-11, Established in Culture from Ehrlich Ascites Carcinoma Cells. Ⅱ: The optimal medium for the cultivation of the standard line, K. and the culture-adaptability of cells after passage through mice
Author
Hamasaki, Mitsuhiko
Abstract
The optimal medium for the cell proliferation of the standard line, K, was investigated and found to consist of 20% bovine serum, 0.4% lactalbumin hydrolysate, 0.4% yeast extract, 0.36% glucose and saline. The cultured cells were retransplanted and passaged intraperitoneally through mice. After 19 transfers through mice, e.g., in 280 days, the cells were re-inoculated into culture and compared in the cellular morphology and in the culture-adaptability with the primary culture of purely mice-passaged Ehrlich carcinoma cells. The former cells adhered readily to the glass surface showing spindle-like form and demonstrated the capacity for the proliferation in culture from the earliest stage of cultivation, while the latter cells adhered hardly to the glass maintaining their round form and revealed little proliferation or underwent degeneration rapidly with days.
Published Date
1964-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume76
Issue
issue4-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
215
End Page
222
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/76/4-6/76_4-6_215/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma