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ID 18238
Eprint ID
18238
FullText URL
Thumnail 77_631.pdf 3.72 MB
Title Alternative
Antimitotic Action of "CORNIN Fractions" Extracted from Rabbit Muscle
Author
Kanao, Hirosi
Abstract
The author succeeded in the extraction of a substance from the skeletal muscle of rabbit designated as "muscle cornin", that markedly inhibits of sea urchin eggs. This substance is dialysable and can be separated into three fractions by DEAE-cellulose column. It has been found that fraction Ⅰ has no inhibitory action on mitosis, while fraction Ⅱ and Ⅲ has marked antimitotic effect, its minimum effective dose are 10(-8)g/ml. The fraction Ⅱ is a nucleoprotein with hypoxanthine or xanthine as the ba se and has the maximum absorbance of ultraviolet spectrum at 249mμ. The fraction Ⅲ is a polypeptide that has no specific maximum absorbancy of ultraviolet spectrum. The muscle cornin not only shows a typical protein wave in polarography but it also proves to be positive to ninhydrin and Liebermann reactions, bromphenol blue stain, and precipitation by trichloroacetic acid. However, it is negative to Biuret, xanthoprotein, Sakaguchi and Molish reactions. As for its sugar reaction, it is also positive to Seliwanoff and phloroglucinol reactions, but is negative to other reactions. Therefore, it has chemical properties that are partially different from those of "corneal cornin". The muscle cornin has no effect whatever on catalase activity and oxygen consumption of tissues. In addition, differing from the corneal cornin, it dose not effect the P/O ratio of rat liver mitochondria. However, just as with the corneal cornin, it dose inhibit the incorporation of (32)p into nucleic acids, especially into DNA and r-RNA of regenerating rat liver, and also it inhibits DNA synthesis. But it shows no effect on RNA synthesis. The discussion was also made on the difference in the properties of muscle cornin from those of other inhibitorsof mitosis, extracted from living tissues.
Published Date
1965-03-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume77
Issue
issue3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
631
End Page
644
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/77/3/77_3_631/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma